方法构建基因工程菌，利用甲醇诱导表达。
MethodThe genetic engineering bacteria was constructed, and induced by methanol.
体外诱导表达后斑点印迹鉴定表位肽的表达。
The expression of the peptide was detected with dot blot assay.
诱导表达的菌体在激光共聚焦显微镜下可发射明亮的绿色荧光。
Moreover the induced bacteria could emit bright green fluorescence by confocal laser scanning microscope.
诱导型一氧化氮合酶的诱导表达往往导致心肌的收缩功能下降。
The up regulation of inducible nitric oxide synthase ( iN OS ) often negatively modulates myocardial function.
诱导表达的菌体在激光共聚焦显微镜下可发射明亮的绿色荧光。
Moreover, the induced bacteria could emit bright green fluorescence by confocal laser scanning microscope.
对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body.
目的：建立一个可诱导表达目的基因的细胞系以研究新基因的功能。
Objective: to establish a stable cell line that can be induced to express a target gene.
全身炎症反应与一些细胞因子如内皮素、肿瘤坏死因子等的诱导表达有关。
SIR is relative to inducing and expression of some of cytokines such as ET and TNF .
目的：构建受强力霉素诱导表达脑啡肽的大鼠星形胶质细胞株(IAST)。
AIM: To construct an immortalized rat astrocyte strain (IAST) expressing enkephalin regulated by Doxycycline.
方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
IPTG诱导表达后，SDS-PAGE显示出现目的条带，与预期结果一致。
Induced under IPTG, the target band appeared as expectlly on SDS-PAGE profile.
目的：构建缺氧诱导表达载体，以介导报告基因在缺氧环境下的特异、高效表达。
Objective: to construct hypoxia-inducible expression vector, which mediated reporter gene to express specially and efficiently in hypoxia environment.
然后诱导表达，用SDS - PAGE及蛋白印迹进行鉴定，然后进行蛋白纯化。
Then it was induced to expression and identified by SDS-PAGE and Western-blotting, and it was purified.
这些基因在水稻不同组织器官具有不同的表达水平，大部分受非生物胁迫诱导表达。
These genes showed variable expression levels in different tissues and organs of rice, and most of them were induced by abiotic stresses.
诱导表达系统可以在特定时间以适当的水平表达目的蛋白，这有利于基因功能的研究。
Inducible expression systems can be used to regulate the expression level of genes of interest in appropriate time according to the aim of the experiments.
在诱导表达优化条件下，该重组菌可产生表达量占细菌总蛋白量30%以上的融合蛋白。
Under the optimized expression condition, the recombinant expressed product is account for over 30% of overall bacterium protein;
目的：构建融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白。
AIM: to construct the recombinant expression vector and to obtain large amount of mouse Cyclin D1 protein fused to PTD.
随着甜瓜采后诱导抗病性研究的深入，对防卫基因的诱导表达分析已成为亟待研究的内容。
As progressing on postharvest induced resistance in muskmelon, it is necessary to analysis the expression of defense genes in treated fruit.
目的观察烧伤小鼠干扰素诱导表达蛋白IFIT1的表达变化，初步探究其表达变化的原因。
Objective To investigate the expression of interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) in burned mice and its mechanism.
表达产物在培养基中，表达的产量依赖于不同的基因序列、载体、宿主和诱导表达条件等因素。
The yield of gene product in culture medium depends on different gene sequences, vectors, hosts and i-uduced condition.
构建牛结核分枝杆菌esat - 6基因的原核表达载体，诱导表达、纯化并初步鉴定该蛋白。
To construct the recombinant prokaryotic expression vector containing the ESAT-6 gene of Mycobacterium Boris, purification, expression the fusion protein identified.
IPTG诱导表达融合蛋白，包涵体蛋白经复性后亲和层析法纯化，ELISA方法测定蛋白活性。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
这些EST中的大多数在对干旱和渗透压胁迫发生反应时是共表达的，而在耐受冷热胁迫时并不被诱导表达。
The majority of these ESTs were co-expressed in response to desiccation and osmotic stress and were generally not induced in response to heat and cold stress.
热诱导型启动子的研究表明，TM2仅提高热诱导的表达强度，而不改变启动子的热诱导表达调控特性。
The effect of TM2 on transcription regulated by heat-inducible promoter indicated that the MAR did not change the regulation pattern but improved the expression level of the heat-inducible promoter.
目的研究沙土鼠海马hsp70基因在缺血-复灌过程中的表达变化，以及不同缺血程度对该基因诱导表达的影响。
Objective To examine the changes of HSP70mRNA levels in gerbil hippocampus during the course of ischemia and reperfusion and the impact of varied ischemic degrees on the induction of this gene.
本文报道了在克隆了小鼠酪氨酸酶基因的基础上，在大肠杆菌表达体系中诱导表达野生型和变异型酪氨酸酶的结果。
Here we reported the cloning of the gene from murine tyrosinase, the expression of wild tyrosinase and variants in E. coli.
随着DD-PCR技术的不断改进，它将在植物抗土壤逆境胁迫诱导表达基因的背景研究中显示出广泛的应用前景。
With improvement of the technique, it will find its wide application in detecting soil stress-induced genes in higher plants.
水稻叶片中的ALDH7B受稻瘟病菌感染、紫外线和机械损伤诱导表达，而未经处理的水稻器官中检测不到表达。
ALDH7B7 expression was induced by blast fungus infection, ultraviolet, mechanical wound in rice leaves and was not detected in untreated rice organs.
CBF基因受到低温、干旱及高盐等非生物胁迫因子诱导表达，除了CBF 4外，其余的CBF基因都不依赖aba。
CBF genes are expressed when induced by abiotic stress factors such as low temperature, drought and high salty, etc. Except for CBF4, other CBF genes are independent on ABA.
CBF基因受到低温、干旱及高盐等非生物胁迫因子诱导表达，除了CBF 4外，其余的CBF基因都不依赖aba。
CBF genes are expressed when induced by abiotic stress factors such as low temperature, drought and high salty, etc. Except for CBF4, other CBF genes are independent on ABA.
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