背景资料概要:目前已有不同的生长和分化因子使用于脊柱融合中诱导骨形成。
Summary of Background Data. Different growth and differentiation factors are currently being used for inducing bone formation in spinal fusion.
结论KD R启动子可以调控融合基因体系选择性地杀伤人胃癌scg7901细胞,并且该体系可以诱导胃癌细胞凋亡。
Conclusion KDR promoter may regulate the CDglyTK fusion gene system to selectively kill the KDR-expressing SCG7901 cells and to induce the cell apoptosis.
精胺能诱导血影膜的融合。
结论PC - 1nf融合蛋白能抑制大鼠系膜细胞增殖、诱导细胞凋亡及减少细胞外基质合成。
Conclusion: PC-1NF fusion protein may inhibit the proliferation, induce apoptosis and decrease ECM production of RMC.
应用细胞融合技术和扫描电镜研究了BK(牛肾)细胞早熟凝集染色体(PCC)和诱导PCC的CHO中期染色体的超微结构。
The ultrastructure of prematurely condensed chromosomes (PCC) in BK cells and the CHO metaphase chromosomes (the PCC inducer) were studied with cell fusion technique and SEM.
结果表明,用此融合蛋白的包涵体免疫豚鼠能诱导产生中和抗体,并对病毒的攻击提供免疫保护。
The results showed that the fusion protein can yield antibodies with high neutralizing activity as well as provide protection against challenge with virulent virus.
人工诱导鱼类细胞融合是细胞工程中的一项重要技术。
The cell fusion of fish is an important technology in cell engineering.
IPTG诱导表达融合蛋白,包涵体蛋白经复性后亲和层析法纯化,ELISA方法测定蛋白活性。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
方法利用聚乙二醇(PEG)诱导SGC7901胃癌细胞、树突状细胞融合,利用HAT、HT筛选培养系统对融合细胞进行筛选培养,获取纯净融合疫苗;
Methods The SGC7901 gastric cancer cells and dendritic cells were induced to be fused by PEG and the pure fusion cells were obtained by selective culture with the HAT/HT culture system.
电场诱导是80年代以来兴起的一种细胞融合技术,在细胞遗传工程实践中具有很大应用价值。
The electric field-induced cell fusion is a new technique for somatic hybridization since 1980's, and has great application potential in cell genetic engineering.
这表明,PEG诱导的鱼类细胞融合具有物种和组织特异性。
This shows that the PEG-induced fish cell fusion has its specificity in relation to species and tissue.
融合蛋白表达量的高低与诱导时的细胞密度,诱导的温度以及培养基有关。在一定范围内与诱导剂的剂量以及诱导时间的长短无关。
The expression level was closely related with the cell density, induction temperature and medium but not with the inductor dosage and the induction period within certain range.
采用体外诱导卵母细胞成熟技术,比较研究了雌核发育银鲫和两性融合发育彩鲫卵母细胞成熟过程中周期蛋白合成和MPF活性变化情况。
The changes in cyclin synthesis and MPF activity during oocyte maturation in vitro in gynogenetic silver crucian carp and amphimictic colored crucian carp were compared.
目的:构建融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白。
AIM: to construct the recombinant expression vector and to obtain large amount of mouse Cyclin D1 protein fused to PTD.
在诱导表达优化条件下,该重组菌可产生表达量占细菌总蛋白量30%以上的融合蛋白。
Under the optimized expression condition, the recombinant expressed product is account for over 30% of overall bacterium protein;
在优化诱导条件后,融合蛋白的表达量可达全菌总蛋白的3 4%。
Under reasonable conditions for induction, amount of the recombinant gene expression products could reach as high as 34% total proteins of the host.
在优化诱导条件后,融合蛋白的表达量可达全菌总蛋白的3 4%。
Under reasonable conditions for induction, amount of the recombinant gene expression products could reach as high as 34% total proteins of the host.
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