整合图形化序列特征，进一步协助您设计引物。
Integrated graphic sequence feature viewer to assist primer design.
根据人的GAPDH基因和毛冠鹿的钾通道基因设计引物。
We designed and synthesized the primers of the human GAPDH gene and the Elaphodus cephalophus potassium channel gene.
对其余的15个微卫星设计引物，有10对引物扩增出目的片段。
Designed the primers of the other 15 new microsatellites, and 10 primers could obtain targeted fragments by PCR amplification.
方法根据已报道的若干物种的NADH脱氢酶亚基4基因(nd4)设计引物。
Methods Primers were designed according to certain species' NADH-ubiquinone oxidoreductase ND4 in which the DNA sequence had been reported.
实验中我们体会到：（1）在设计引物时，要注意避开UGA，可用UGG代替。
The authors considered:(1)To design primers attention must be paid to evade UGA and to take place of UGG.
方法：根据MBL基因序列设计引物建立MBL基因点突变检测方法即PCR -RFLP ；
Methods:The method for MBL point mutation detection(PCR-RFLP) was Established with self-designed primers according to MBL genomic sequence;
根据计算机克隆的ZNF322基因序列设计引物，从人类胚胎心脏文库中克隆了ZNF322基因。
The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.
主要进行了以下工作：（1）根据GENBANK公布的羊痘病毒基因组序列设计引物，利用PCR克隆GTPV-TY株TK基因和P32基因；
The following work were done in this study: (1)TK and P32 gene of GTPV-TY were cloned by PCR with primer pairs designed by information published on GENBANK;
根据NCBI上发表的BVE2490株的基因组序列，将其与其他疱疹病毒科病毒的基因组进行比对，找出其中有差异的序列，根据这些序列设计引物。
We compare the genome of BV E2490 in NCBI with other herpes virus to find the sequence with diversity, and try to design primers in the sequences.
序列信息具有实际意义-如，通过设计PCR引物，我们可以快速对这种病毒做出诊断，从而扩宽和加快监控。
Sequence information can have practical significance - for example, in designing PCR primers, to make rapid diagnosis tests that broaden and speed up surveillance.
本实验利用种属相似性设计了一对引物。
In the experiment, a pair of primers was designed by species similarity.
本研究设计并合成了一对引物。
根据苯酚羟化酶基因高度保守序列设计一对该基因的特异引物。
A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence.
在应用这一方法时，我们最关心以下三个方面：敏感性，忠实性和引物设计的方便。
Our main concern while using this method involves sensitivity, fidelity and the ease of primer design.
进一步工作是设计特异性引物，将其转化为SCAR标记。
Next work is to design the differential primers and transvert it to SCAR.
经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。
We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.
新方法扩展了“通用引物”的适用范围，并为引物设计和一些其它基因的PCR放大提供了思路。
Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
根据菌属基因序列设计PCR引物，提取基因组dna进行PCR快速检测，然后进行测序比对。
According to the bacteria gene sequence, we also designed PCR primer, and collected genome DNA for PCR rapid test, then tested and contrasted sequence.
方法根据HCVH病毒株序列设计、合成序列特异性的引物。
Methods Sequence specific primers were designed and synthesized according to the HCV H strain of virus sequence.
方法设计相应的引物，用RT _ PCR，基因克隆，DNA序列分析技术。
MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used.
方法根据APP的基因序列，设计并合成引物和荧光标记探针。
Methods According to the specific sequence of APP genes, the primers and the fluorogenic probe were designed and synthesized.
引物设计是RAMP和REMAP标记分析的关键，二者都使用锚定的微卫星序列引物。
The primer design is a key step for RAMP and REMAP, in which anchored simple sequence repeat primers were used.
引物设计是P CR技术中最重要的一环。
PCR-primer design is the most important step in polymerase chain reaction (PCR) technique.
成功进行简并PCR的关键是设计好两组引物库和对反应条件进行优化。
The optimization of reaction conditions and the primer design are the key factors for the success accomplishment of degenerate PCR.
图1抗性基因STV11引物设计策略红色表示引物结合位点，蓝色表示序列差异位点。
Fig. 1. Design strategies for STV11 marker. Primer binding sites are shown in red, sites with different sequence are shown in blue.
这里，给出了亚硫酸氢盐转换，引物设计和PCR条件优化的一个详细的实验方案。
Here, a detailed protocol for bisulphite conversion, primer design, and optimization of PCR conditions is given.
设计了特异性较好的引物。
重叠延伸PCR技术成功的关键是重叠互补引物的设计。
The overlap primers design is the key factor for the successful accomplishment of SOE PCR.
方法设计一对寡核苷酸引物，其上游引物为突变引物。
Methods A pair of primer was designed and a mismatch nucleotide was introduced in its upstream primer.
方法设计一对寡核苷酸引物，其上游引物为突变引物。
Methods A pair of primer was designed and a mismatch nucleotide was introduced in its upstream primer.
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