结论兔角膜缘上皮细胞可以在体外培养、冻存和复苏。
Conclusion the limbal corneal epithelial cells of rabbit could be cultivated, cryopreserved and resuscitated in vitro.
目的建立兔角膜缘上皮细胞体外培养、冻存和复苏的方法。
Objective to establish a method of cultivating limbal corneal epithelial cells of rabbit in vitro and to study its cryopreservation and resuscitation.
目的：建立体外培养兔角膜缘上皮细胞的方法，观察其体外生长的生物学特性。
Objective:To establish a method for culturing limbal corneal epithelial cells of rabbit and to observe the growth characteristics of the cultured cells in vitro.
目的：用体外培养的角膜缘上皮细胞联合人羊膜的方法，行自体移植治疗角膜缘干细胞完全缺乏的兔眼。
Objective: to study in vitro cultivated limbal epithelial cells autograft with amniotic membrane grafts for total limbal stem cell deficiency of rabbit eyes.
结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
伤口均在角膜周边部或角膜缘，病因是手术缝合创口对合不佳，结膜或角膜上皮细胞植入虹膜组织。
The iris cysts were mainly caused by inadequate healing of the wound and the implantation of epithelium of either conjunctiva or cornea.
采用这些标记复合体，可将角膜缘干细胞群与其他上皮细胞区分。
With these markers complex, it is easy to distinguish limbal stem cells from other epithelial cells in cornea.
新鲜板层角膜含健康的角膜缘干细胞和角膜上皮细胞，可迅速修复损伤的角膜表面。
Fresh lamellar cornea contains health corneal limbus stem cells and epithelial cells, it can repair the trauma corneal surface soon.
结果10例新鲜角膜缘上皮组织培养后，7例有上皮细胞生长，1周形成细胞单层；
Results Cells growth occ urred in7of10pieces of fresh limbal tissue and formed monolayer cells in1wee k.
角膜缘干细胞的发现是十几年来眼科最重要的进展之一，现已公认，角膜缘干细胞是角膜上皮细胞再生的最终源泉。
The discovery of limbal stem cell is one of the most important advances in ophthalmologic field. It is now accepted that limbal stem cell is the ultimate source of corneal epithelium regeneration.
角膜缘干细胞的发现是十几年来眼科最重要的进展之一，现已公认，角膜缘干细胞是角膜上皮细胞再生的最终源泉。
The discovery of limbal stem cell is one of the most important advances in ophthalmologic field. It is now accepted that limbal stem cell is the ultimate source of corneal epithelium regeneration.
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