目的了解角膜上皮干细胞的生长特性。
Objective To explore the growth features of corneal epithelial stem cells in vitro.
结论胎儿角膜上皮干细胞呈现由全层向基底层，再向缘部逐渐迁移的分布规律，且超微结构较幼稚。
Conclusion the migration of corneal epithelial stem cells in the human fetuses was from the whole layers to basal layer and confined to limbus region finally, and their ultrastructure was immature.
角膜缘干细胞是角膜上皮更新的源泉，维持角膜完整性。
Corneal limbus stem cells are the source of corneal epithelial renewal and they help maintain the integrity of cornea.
新鲜板层角膜含健康的角膜缘干细胞和角膜上皮细胞，可迅速修复损伤的角膜表面。
Fresh lamellar cornea contains health corneal limbus stem cells and epithelial cells, it can repair the trauma corneal surface soon.
结论采用无饲养层细胞培养体系构建自体角膜上皮组织，移植后可以成功修复角膜缘干细胞缺失的角膜上皮。
Conclusion the reconstructed rabbit corneal epithelium hi vitro with a free-feeding layer culture system could be autografted to repair total corneal limbal stem cells deficiency.
结论角膜及角膜缘表层上皮较为成熟分化，而角膜缘基底细胞具有未分化细胞的特征，很可能是干细胞的部位。
ConclusionThe superficial cells of limbus and cornea are of mature status, while limbal basal cells show some undifferentiated feature, which indicates the location of stem cells.
目的采用无饲养层细胞培养体系构建家兔角膜上皮组织，自体移植后观察其对角膜缘干细胞缺失的治疗效果。
Objective to reconstruct rabbit corneal epithelium in vitro with a free-feeding layer culture system and autograft to treat limbal stem cells deficiency.
采用这些标记复合体，可将角膜缘干细胞群与其他上皮细胞区分。
With these markers complex, it is easy to distinguish limbal stem cells from other epithelial cells in cornea.
目的：用体外培养的角膜缘上皮细胞联合人羊膜的方法，行自体移植治疗角膜缘干细胞完全缺乏的兔眼。
Objective: to study in vitro cultivated limbal epithelial cells autograft with amniotic membrane grafts for total limbal stem cell deficiency of rabbit eyes.
角膜上皮的增生与更新依赖于角膜缘干细胞，角膜缘干细胞缺乏可导致视力障碍。
The proliferation and refreshment of corneal epithelium depends on corneal limbal stem cells. Corneal limbal stem cell deficiency can lead to visual disorder.
角膜缘部上皮基底层的干细胞具有细胞更新和组织再生的功能，而眼部表面疾病则是干细胞缺乏的结果。
The stem cells of the basal layer of the limbal epithelium have function of cell renewal and tissue regeneration, and ocular surface disorders result from lack of the stem cells.
角膜缘干细胞的发现是十几年来眼科最重要的进展之一，现已公认，角膜缘干细胞是角膜上皮细胞再生的最终源泉。
The discovery of limbal stem cell is one of the most important advances in ophthalmologic field. It is now accepted that limbal stem cell is the ultimate source of corneal epithelium regeneration.
角膜缘干细胞的发现是十几年来眼科最重要的进展之一，现已公认，角膜缘干细胞是角膜上皮细胞再生的最终源泉。
The discovery of limbal stem cell is one of the most important advances in ophthalmologic field. It is now accepted that limbal stem cell is the ultimate source of corneal epithelium regeneration.
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