方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
并通过荧光定量PCR研究其在外施五种激素的条件下的胶乳中的表达谱。
The real time quantitative PCR is applied to research the expression trend of these genes under five different hormones.
而利用表达荧光蛋白的新模型就可以比较准确方便地对肺和腹腔转移灶进行定量。
The new models are very useful for detecting and quantitating small lung metastasis in a objective way, and the abdomen metastasis could be quantitated now.
采用实时荧光定量PCR鉴定高表达基因。
Differentially expressed genes were identified by real-time quantitative PCR.
结果显示，1．荧光定量RT干CR方法是一种定量检测基因表达丰度的有效方法，具有很好的临床应用前景；
Results showed that 1. fluorescent quantitative RT-PCR was an efficient quantification method to detect the gene expression level and has a prospecting application in clinic;
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
通过表达谱芯片检测药物作用后的基因表达差异，对差异表达基因进行生物信息学研究并结合实时荧光定量pcr分析。
Then the GO and KEGG Pathway enrichment analysis were carried as well as the functional analysis and real time RT-PCR validation of two important genes.
实时荧光定量PCR结果表明，该基因在受到条锈菌诱导下，在亲和组合中表达趋势明显下调，而在非亲合组合中的表达趋势比较稳定。
The results of real-time PCR analysis revealed that TAFFC transcripts were steady in incompatible interaction of wheat and stripe rust, whereas down-regulated in compatible interaction.
实时荧光定量PCR试验表明，FABP4基因在160和200日龄绵羊背最长肌中的表达量明显高于90日龄背最长肌的表达量（P< 0.05）；
The real time RT-PCR revealed that expressions of FABP4 in longissimus muscle from 160 and 200-day's sheep were higher than those from 90-day's (P<0.05), expectively.
实时荧光定量PCR试验表明，FABP4基因在160和200日龄绵羊背最长肌中的表达量明显高于90日龄背最长肌的表达量（P< 0.05）；
The real time RT-PCR revealed that expressions of FABP4 in longissimus muscle from 160 and 200-day's sheep were higher than those from 90-day's (P<0.05), expectively.
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