克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
Clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
测序表明读码框架正确后，将重组子分别转入表达宿主菌e。
After the reading frame was confirmed correct by sequencing, the recombinant plasmid was transformed into expression host e.
结论构建了含人pd - 1基因重组逆转录病毒的载体，筛选出稳定表达人PD - 1分子的L929细胞株。
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.
目的克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
Objective To clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
目的克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
Objective To clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
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