该表达载体的构建为获得大量NS1蛋白进行功能研究及抗体制备提供了基础。
Construction of the prokaryotic expression vector provided a foundation for further studying the function of NS1 protein and preparation of NS1 antibody.
完成了NKG2D的原核表达载体的构建，表达并纯化了重组NKG2D蛋白。
The NKG2D prokaryotic expression vector was successfully constructed. The recombinant NKG2D is expressed and purified.
HBV靶向核糖核酸酶真核表达载体的构建及其在2.2.15细胞内的表达。
Construction of HBV targeted ribonuclease and its expression in 2.2.15 cell line.
该表达载体的构建为进一步研究人id4基因的启动子活性及表达调控奠定了基础。
The construction of expression vector of human ID4 promoter provides a platform for further researches on promoter activity and expression regulation of human ID4 gene.
人血管生成素-1基因的克隆及表达载体的构建，为进一步研究其功能、活性和应用奠定了基础。
The cloning of human angiopoietin 1 gene and construction of it's expression vectors lay a good foundation of further study on the function, biological activity and application.
结论：原核表达载体的构建、重组蛋白的表达、纯化及多克隆抗体的制备为今后研究该蛋白的功能提供了良好的基础。
CONCLUSION: Recombined prokaryotic expression vector, the purified protein and prepared polyclonal antibody were the necessary materials for further study of this protein.
结论野生型和突变型PRPF31基因真核表达载体的构建，为研究PRPF31基因突变引起视网膜色素变性的机制奠定了基础。
Conclusion Construction of the eukaryotic expression vectors of wild type and mutant PRPF31 genes is basic work for research on the mechanisms of retinitis pigmentosa caused by PRPF31 mutation.
重点讨论了各种检测方法在实际中的应用，百合无症病毒提取过程中需要解决的问题，外壳蛋白基因在大肠杆菌中表达载体的构建。
The discussion was mainly focused on the application of these detection methods, the problem result in the virus extraction progress, and the construction of expression plasmid having LSV CP gene.
本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
构建了鹦鹉热衣原体主要外膜蛋白的表达载体。
An expressing vector of major outer-membrane protein of Chlamydia psittaci was constructed.
目的构建狂犬病病毒糖蛋白基因的真核细胞表达载体，并在COS - 7细胞中表达。
Objective To construct a recombinant plasmid for expression of rabies virus glycoprotein in COS-7 cells.
目的：构建缺氧诱导表达载体，以介导报告基因在缺氧环境下的特异、高效表达。
Objective: to construct hypoxia-inducible expression vector, which mediated reporter gene to express specially and efficiently in hypoxia environment.
为构建其启动外源基因的质粒表达载体作准备。
It was therefore preparation for constructing an expression plasmid vector to express exogenous genes.
目的：构建人死亡受体5 (DR5)真核表达载体，转染NS - 1细胞，建立稳定转染的NS - 1细胞系。
Objective: to construct eukaryotic expression vector of human death receptor (DR5) and transfect NS-1 cells to establish stable NS-1 cell line.
实验结果表明，以TK基因构建的重组载体质粒可用于外源基因的重组表达研究。
The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.
目的构建人il -12的高效表达载体。
Objetive to construct human IL 12 efficient expression vector.
目的：构建高免疫原性的抑制素真核表达载体。
Objective: to construct inhibin expression vector with high immunogenicity.
目的构建以绿色荧光蛋白（GFP）为报告基因的酿酒酵母表达载体。
Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
目的构建木瓜凝乳蛋白酶的表达载体，并在大肠杆菌中表达。
Objective To construct recombinant plasmid for expression of chymopapain in E.
结论已成功地构建了人胰岛素基因的植物细胞表达载体。
Conclusion The vector for expression of human insulin in plant cells was successfully constructed.
目的：构建丙型肝炎病毒(HCV)全长及3种不同缺失突变的核心蛋白基因的原核表达载体，并在大肠杆菌中表达。
AIM: to construct the recombinant plasmids expressing full-length HCV core protein gene and 3 different deletion mutated hepatitis core protein genes and to express them in E. coli.
结果：成功构建了DDR2/EGFP融合表达载体，进一步的分析也证明此融合表达载体能在细胞中正确表达并可以被配体所激活。
RESULTS: DDR2/EGFP fusion plasmid was successfully constructed. Further analysis also demonstrated that the fusion protein has similar expression and activation pattern with wild type ones.
目的构建水痘-带状疱疹病毒(VZV)糖蛋白e的真核表达载体。
Objective to construct the eukaryotic expression vector of varicella-zoster virus (VZV) glycoprotein e.
将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上，构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
结论构建了人瘦素的哺乳动物细胞表达载体，并成功地在COS 7细胞中获得重组人瘦素的分泌表达。
Conclusion Cloning and expression recombinants of human leptin are obtained and secretive recombinant human leptin is successfully expressed in COS-7 cells.
结论已成功构建了人hsp70与MAGE - 4抗原表位基因的原核表达载体，为疫苗研究提供了依据。
Conclusion the prokaryotic expression vector for HSP70 and MAGE-4 epitope genes was successfully constructed, which provided a basis for the development of vaccine.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
目的构建人高迁移率族蛋白1（HMGB1）编码基因的表达载体，获得纯化的重组蛋白，研究其生物学功能。
Objective To construct the recombinant vector, the expression of human HMGB1 code gene and to study its function.
目的构建人高迁移率族蛋白1（HMGB1）编码基因的表达载体，获得纯化的重组蛋白，研究其生物学功能。
Objective To construct the recombinant vector, the expression of human HMGB1 code gene and to study its function.
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