结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
本文简要介绍了近年来在植物表达载体构建方面所采用的一些新策略，这些策略有助于增强外源基因的表达水平、提高生物工程体的安全性。
This article reviews the new strategies currently used in construction of plant expression vector for plant genetic engineering. These strategies are important for obtaining better expression of fo...
本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
构建了鹦鹉热衣原体主要外膜蛋白的表达载体。
An expressing vector of major outer-membrane protein of Chlamydia psittaci was constructed.
人血管生成素-1基因的克隆及表达载体的构建，为进一步研究其功能、活性和应用奠定了基础。
The cloning of human angiopoietin 1 gene and construction of it's expression vectors lay a good foundation of further study on the function, biological activity and application.
目的构建反义MBD1基因片段真核表达载体，为研究MBD1基因功能提供工具。
Objective to construct eukaryotic expression vector of antisense MBD1 gene fragment and to provide a tool for studying MBD1 gene function.
结论野生型和突变型PRPF31基因真核表达载体的构建，为研究PRPF31基因突变引起视网膜色素变性的机制奠定了基础。
Conclusion Construction of the eukaryotic expression vectors of wild type and mutant PRPF31 genes is basic work for research on the mechanisms of retinitis pigmentosa caused by PRPF31 mutation.
目的：构建缺氧诱导表达载体，以介导报告基因在缺氧环境下的特异、高效表达。
Objective: to construct hypoxia-inducible expression vector, which mediated reporter gene to express specially and efficiently in hypoxia environment.
目的构建水痘-带状疱疹病毒(VZV)糖蛋白e的真核表达载体。
Objective to construct the eukaryotic expression vector of varicella-zoster virus (VZV) glycoprotein e.
目的：构建人死亡受体5 (DR5)真核表达载体，转染NS - 1细胞，建立稳定转染的NS - 1细胞系。
Objective: to construct eukaryotic expression vector of human death receptor (DR5) and transfect NS-1 cells to establish stable NS-1 cell line.
目的构建大肠杆菌K99菌毛表达载体。
实验结果表明，以TK基因构建的重组载体质粒可用于外源基因的重组表达研究。
The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.
该表达载体的构建为获得大量NS1蛋白进行功能研究及抗体制备提供了基础。
Construction of the prokaryotic expression vector provided a foundation for further studying the function of NS1 protein and preparation of NS1 antibody.
目的构建人il -12的高效表达载体。
Objetive to construct human IL 12 efficient expression vector.
完成了NKG2D的原核表达载体的构建，表达并纯化了重组NKG2D蛋白。
The NKG2D prokaryotic expression vector was successfully constructed. The recombinant NKG2D is expressed and purified.
为构建其启动外源基因的质粒表达载体作准备。
It was therefore preparation for constructing an expression plasmid vector to express exogenous genes.
目的构建重组人瘦素哺乳细胞表达载体并在COS 7细胞表达重组人瘦素。
Objective to construct recombinant human leptin mammalian cell expression vector, and to express recombinant human leptin in COS-7 cells.
目的克隆人TRAIL基因，构建其原核表达载体。
To clone gene of the TRAIL and construct its prokaryotic expression vector.
目的克隆人热休克蛋白70 (HSP70)基因，构建其原核高效表达载体。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
目的：构建高免疫原性的抑制素真核表达载体。
Objective: to construct inhibin expression vector with high immunogenicity.
目的：构建抗腮腺炎病毒抗体轻链基因重组表达载体。
Objective: To construct the recombinant expression vector of genes encoding light chain of antibody against Mumps Viruses.
目的构建木瓜凝乳蛋白酶的表达载体，并在大肠杆菌中表达。
Objective To construct recombinant plasmid for expression of chymopapain in E.
目的构建狂犬病病毒糖蛋白基因的真核细胞表达载体，并在COS - 7细胞中表达。
Objective To construct a recombinant plasmid for expression of rabies virus glycoprotein in COS-7 cells.
目的：构建CD 147反义rna表达质粒载体，探索治疗骨肉瘤侵袭和转移的新方法。
AIM: to construct eukaryotic antisense RNA expression vector of CD147 and probe into a new method to treat invasion and transfer of osteosarcoma.
将其全长片段与GUS基因连接构建植物表达载体并转化烟草。
A plant expression vector was then constructed by ligating the cloned fragment to GUS gene, used for transformation of tobacco.
目的构建弓形虫多表位基因（TGMG）植物表达载体。
Objective To construct the plant expression vectors containing the multiepitope gene of Tazoplasma gondii (TGMG).
通过构建表达载体在大肠杆菌中得到了表达。
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
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