结果突变基因重组表达质粒构建正确；
Results The recombinant plasmid with mutant gene was constructed correctly.
目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
目的：构建弓形虫棒状体蛋白（ROP2 ）基因重组质粒并在大肠杆菌中表达，用于筛选弓形虫新的诊断抗原和疫苗分子。
Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen (WHcAg) and prepare polyclonal antibodies.
实验结果表明，以TK基因构建的重组载体质粒可用于外源基因的重组表达研究。
The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.
目的：克隆大鼠神经营养因子4全长基因，构建真核细胞表达质粒。
AIM: to clone rat neurotrophin-4 (NT-4) total gene and construct expression plasmid for prokaryotic cells.
构建含蛋白转导结构域(PTD)与脑源性神经营养因子(BDNF)融合基因的质粒，并在大肠肝菌中表达。
To construct a recombinant plasmid containing protein transduction domain (PTD) and brain derived neurotrophic factor (BDNF) fusion gene and express in e.
为构建其启动外源基因的质粒表达载体作准备。
It was therefore preparation for constructing an expression plasmid vector to express exogenous genes.
目的：构建CD 147反义rna表达质粒载体，探索治疗骨肉瘤侵袭和转移的新方法。
AIM: to construct eukaryotic antisense RNA expression vector of CD147 and probe into a new method to treat invasion and transfer of osteosarcoma.
构建小鼠il -4截短型基因真核表达质粒，表达小鼠il - 4受体拮抗体蛋白。
To clone mouse interleukin 4 (mIL-4) truncated gene, construct its eukaryotic expression plasmid pFB-mIL4 and express the truncated protein (murine IL-4 receptor antagonist).
序言真核表达质粒的构建和转染是目前医学研究中常用的分子生物学手段。
Introduction The construction and transfection of eukaryotic expression plasmid are commonly molecular biology method used in medical research.
目的：构建食管癌相关基因2ECRG2原核表达质粒，在大肠杆菌e。
Objective: to construct a prokaryotic expression vector containing esophageal cancer related gene 2 ECRG2 and observe its expression in Escherichia coli e.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
结论：高免疫原性的抑制素表达质粒的构建为利用抑制素基因免疫技术诱导单胎动物生多胎奠定了基础。
Conclusion: the fusion gene expression vector was successfully constructed, and it set up the basis of inhibin gene immunization to induce multiple bear for single birth animals.
目的：构建基质金属蛋白酶9靶向的RNA干扰质粒载体，观察其对小鼠巨噬细胞基质金属蛋白酶9基因表达的沉默作用。
AIM: to construct plasmid vector of RNA interference specific for matrix metalloproteinases (MMP) -9 and observe the silencing effect on gene expression of MMP-9 in macrophage of mice.
目的构建癌胚抗原（CEA）与热休克蛋白70（HSP70）的真核双表达质粒，并检测其在体外的表达。
Objective To construct the eukaryotic coexpression plasmid encoding carcinoembryonic antigen(CEA) and heat shock proteins 70 (HSP70), then detect the expression of the plasmid in vitro.
目的：利用已有质粒构建带GF P报告基因的启动子鉴定质粒，并用此质粒鉴定丙型肝炎病毒5非翻译区(HCV 5'-UTR)启动基因表达的活性。
Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
相应地将构建的表达质粒导入变铅青链霉菌TK24中获得五株基因工程菌株。
These plasmids were introduced into S. lividans TK24, respectively and five genetic engineering strains were constructed.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
目的构建人胰岛素样生长因子1 (IGF - 1)的真核细胞表达质粒。
Objective to construct eukaryotic expression plasmid for human insulin-like growth factor 1 (IGF-1).
结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。
Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
并构建针对集聚蛋白的反义质粒，从而进一步研究下调集聚蛋白的表达对淋巴细胞活化的影响。
Constructed antisense plasmids were used to explore function of agrin in the proliferation of lymphocyte activation.
结论成功构建了重组人FHIT真核表达质粒。
Conlusion The human FHIT recombinant eukaryotic expression plasmid was constructed successfully.
目的：构建人血清及糖皮质激素诱导型蛋白激酶（SGK1）超表达载体质粒，为SGK1的体外研究提供有效的工具。
Objective:To construct plasmid with overexpressed human SGK1 to provide a useful tool for its studies in vitro.
结果构建了具有正确基因序列的CFP10重组表达质粒，重组蛋白在大肠杆菌BL21（DE3）中以包涵体形式表达。
The recombinant CFP10 protein was expressed in inclusion body in E. coli BL21(DE3), and the target gene had been cloned into host bacterium.
将筛选的抗体模拟肽与TSST - 1突变体基因融合或化学耦联，构建重组表达质粒。
Through crosslinking the specific peptide and the gene of TSST-1, we got the recombination plasmid.
方法：用脂质体转染携带PTTG基因的表达质粒，构建过表达PTTG基因的垂体细胞株，观察细胞形态。
Methods: Transfect plasmid which carries PTTG gene use liposome, constructs the pituitary cell line which high express PTTG gene, observe the cell form.
为了确定这个相互作用，我们构建了能表达带有GST和His6标签的全长Arx的原核表达质粒。
To confirm this interaction, we expressed full-length of mouse Arx tagged with either His6 or GST in bacteria.
为了确定这个相互作用，我们构建了能表达带有GST和His6标签的全长Arx的原核表达质粒。
To confirm this interaction, we expressed full-length of mouse Arx tagged with either His6 or GST in bacteria.
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