目的：优化融合表达抑肽酶基因工程菌的发酵条件，分离纯化抑肽酶。
Aim: to optimize the fermentation conditions of engineering bacteria expressing fusion proteins composed of aprotinin and to purify aprotinin.
利用GUS基因的酶学特性，进行瞬时表达研究，目的是在短期内优化转化条件。
Utilizing enzymology trait of GUS gene, we did instantaneous expression experiments so that we could optimize the transgenic conditions in short run.
对大肠杆菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。
Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E.
最小的控制界优化数学模型，最后给出主要研究过程、最优控制下限的数学表达式及其适用条件。
Major parts of the researching process, the formula for the optical control limits and the application condi.
通过对各种条件的优化探索，建立了准确和简单易行的检测药用植物的功能基因表达的平台。
Through the optimization and exploration of various conditions, an exact and easy operation platform is successfully constructed for detecting functional gene expression of officinal plant.
目的：优化重组铜绿假单胞菌外毒素A（PEA）基因工程菌的发酵条件，实现PEA的高效表达。
Objective:To optimize the fermentable conditions of recombinant E. coli BL21 for high level expression of PEA.
对人hsp70基因的克隆、表达，并对其进行表达条件的优化，为研究HSP70的结构、功能与临床应用提供了必要条件。
The human hsp70gene has been cloned and expressed in high level. This study provides a necessary condition for research on structure, function and clinical application of hHSP70.
同时对表达条件进行了优化，其表达量可达16.28%。
The expression capacity could be increased to 16.28% by optimizing expression conditions.
为了提高目标蛋白的表达水平，进行了条件优化，诸如培养基、诱导时间和诱导剂量。
The different conditions, such as culture media, induced time and dosage of IPTG, were used to improve the target protein expression level.
方法：通过实验条件的优化确定了适合GM-CSF表达的诱导温度、诱导时间和培养基等。
Methods:The experiment condition was optimized to determine the suitable inducement temperature, time and media of CM-CSF, etc.
在诱导表达优化条件下，该重组菌可产生表达量占细菌总蛋白量30%以上的融合蛋白。
Under the optimized expression condition, the recombinant expressed product is account for over 30% of overall bacterium protein;
通过优化原核表达条件，确定了原核表达的最佳诱导时间和诱导剂浓度。
After optimizing prokaryotic expression conditions, we determined the optimum inducement time and concentration of IPTG.
结论成功用大肠杆菌表达系统表达创伤弧菌溶细胞素并对其纯化、复性条件进行优化。
These results indicate that the cytolysin gene from V. vulnificus successfully express in the E. coli system.
将表达的重组E2蛋白纯化后作为ELISA包被抗原，建立了一种基于重组E2蛋白的间接ELISA方法，并对此方法的反应条件进行了优化。
An indirect ELISA was developed and optimized with the purified recombinant E2 protein as coating antigen. The optimal conditions of ELISA were determined as follows.
在优化诱导条件后，融合蛋白的表达量可达全菌总蛋白的3 4%。
Under reasonable conditions for induction, amount of the recombinant gene expression products could reach as high as 34% total proteins of the host.
将重组质粒转化至大肠杆菌宿主中，对目的蛋白进行诱导表达，并对诱导表达条件如温度、IPTG浓度等进行了优化。
The expression condition of plasmid pET-tmcC has been explored, but at all the testing temperature, IPTG concentration and different hosts protein expression was not found.
分别以重组蛋白疫苗、商品疫苗和PBS免疫仔猪，优化检测免疫抗体水平的间接ELISA条件，通过比较三个免疫组的抗体水平来评价表达蛋白作为免疫抗原的有效性。
Piglets of each groups were vaccinated respectively with recombinant protein vaccine, commodity vaccine and PBS as contrast. The serum IgG anti-protein of each groups were detected by indirect ELISA.
分别以重组蛋白疫苗、商品疫苗和PBS免疫仔猪，优化检测免疫抗体水平的间接ELISA条件，通过比较三个免疫组的抗体水平来评价表达蛋白作为免疫抗原的有效性。
Piglets of each groups were vaccinated respectively with recombinant protein vaccine, commodity vaccine and PBS as contrast. The serum IgG anti-protein of each groups were detected by indirect ELISA.
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