目的构建天花粉蛋白(TCS)突变体基因，并进行表达及纯化。
Objective To construct and express a trichosanthin (TCS) gene mutant and purify the expressed product.
结论：成功地克隆、表达及纯化NBEAL1蛋白，NBEAL1蛋白在人脑胶质瘤组织中的表达与其恶性程度呈负相关。
Conclusion: the NBEAL1 protein has been successfully cloned, expressed and purified. NBEAL1 protein expression in glioma tissues is negatively associated with the pathological grades of glioma.
方法：在表达及分离纯化VEGF的基础上，成功地制备了VEGF抗体，以夹心法测定vegf。
Methods: Based on isolation, purification and expression of VEGF, successfully prepared anti VEGF antibody and developed a sandwich radioimmunoassay for VEGF.
然后诱导表达，用SDS - PAGE及蛋白印迹进行鉴定，然后进行蛋白纯化。
Then it was induced to expression and identified by SDS-PAGE and Western-blotting, and it was purified.
简要介绍了去垢剂在膜蛋白研究中的最新应用进展，步及去垢剂在膜蛋白离体表达、分离和纯化、以及结构研究中的应用。
In this article, the latest bibliography were reviewed related to applications of detergents to in vitro expression, purification, and structural investigation of membrane proteins.
羧酸酯酶A2表达纯化及特征分析为其应用奠定了基础。
The study on the expression and characterization of carboxylesterase A2 in E. coli is more useful for its future application.
结果纯化后的颌下腺腺泡细胞呈铺路石样形态，原代至第5代培养基及细胞内均可检测到淀粉酶表达；
Result Cobblestone-like submandibular gland acinar cells were found after purification, and amylase was successfully detected both in culture medium and cells of passage 0-5 respectively.
利用镍柱亲和层析对表达的重组蛋白进行纯化，经SDS-PAGE及薄层扫描分析表明，重组蛋白的纯度在90%以上；
The recombinant protein was purified through Ni-chelating affinity chromatography, and the purity was above 90% explained by SDS-PAGE gel scan analysis.
结论：原核表达载体的构建、重组蛋白的表达、纯化及多克隆抗体的制备为今后研究该蛋白的功能提供了良好的基础。
CONCLUSION: Recombined prokaryotic expression vector, the purified protein and prepared polyclonal antibody were the necessary materials for further study of this protein.
目的构建针对APRIL的免疫抑制分子，并对其原核表达蛋白进行纯化及活性筛选。
Objective To construct, express, purify and screen immunosuppressive molecule against human soluble APRIL (a proliferation-inducing ligand).
目的构建表达P30蛋白抗原表位的重组质粒及工程菌，获得纯化的P30蛋白抗原表位，用于检测弓形虫特异性抗体。
Objective To construct a recombinant plasmid containing P30 gene of Toxoplasma gondii and obtain recombinant protein Methods P30 gene fragment was amplified from genomic DNA of T.
目的构建表达P30蛋白抗原表位的重组质粒及工程菌，获得纯化的P30蛋白抗原表位，用于检测弓形虫特异性抗体。
Objective To construct a recombinant plasmid containing P30 gene of Toxoplasma gondii and obtain recombinant protein Methods P30 gene fragment was amplified from genomic DNA of T.
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