然后在该培养基配方的基础上进行摇瓶分批补料发酵试验，确定最佳补料方案。
The fed-batch fermentation method was studied by flask culture based on the optimal medium.
采用正交试验设计方法，确定最佳发酵培养基配方，并考察了中间补料方案。
The optimization of the medium was carried out by using orthogonal analytical method.
采用正交试验设计方法，确定最佳发酵培养基配方，并考察了中间补料方案。
The optimization of the medium was carried out by using orthogonal analytical method.
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