用融合蛋白表达系统对该半分子进行大肠杆菌表达。
Then, a fusion protein expression system was used to express the TNF half molecule.
采用SDS - PAGE凝胶电泳对融合蛋白表达产物的分子量进行了验证。
SDS-PAGE was used to verify the molecular weight of fusion protein product.
目的：获得RECK基因GFP融合蛋白表达载体并使其在脑胶质瘤细胞SHG44中表达。
Objective: To obtain GFP-RECK expression vector and to express it in glioma cell line SHG44.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
结果从活化的人白细胞中克隆到IDO基因编码区全长，并构建了IDOEGFP融合蛋白表达载体。
Results The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed.
融合蛋白表达量的高低与诱导时的细胞密度，诱导的温度以及培养基有关。在一定范围内与诱导剂的剂量以及诱导时间的长短无关。
The expression level was closely related with the cell density, induction temperature and medium but not with the inductor dosage and the induction period within certain range.
结论：成功构建了人ip - 10融合蛋白表达载体，并纯化得到具有活性的IP - 10融合蛋白，为进一步研究IP - 10的功能提供了重要的实验材料。
CONCLUSION: We successfully construct IP-10 fusion protein expressing vector and get the fusion protein with high bioactivity, which provides essential materials for the future studies on IP-10.
以EDDIE为融合蛋白是在大肠杆菌中高效表达抗菌肽的一种好方法。
This method provides an excellent way for high expression of antimicrobial peptides when fused with EDDIE.
目的：表达和纯化半乳糖凝集素- 1融合蛋白。
Objective: To express and purify the fusion protein of galectin-1.
结论GRA7基因在大肠埃希菌中以gst融合蛋白的形式得到表达。
Conclusion The GRA7 gene may be expressed as a GST fusion protein in E. coli.
构建含蛋白转导结构域(PTD)与脑源性神经营养因子(BDNF)融合基因的质粒，并在大肠肝菌中表达。
To construct a recombinant plasmid containing protein transduction domain (PTD) and brain derived neurotrophic factor (BDNF) fusion gene and express in e.
目的高密度培养重组细菌和高效表达重组幽门螺杆菌尿素酶a融合蛋白。
Cell density cultivation and high level expression of recombinant urease a fusion protein in Helicobacter pylori.
目的：表达人免疫相关鸟苷三磷酸酶基因(IRGM) a全长融合蛋白，制备高质量的兔抗人IRGM多克隆抗体。
Aim: To express the whole length fusion protein of human IRGMa and prepare high quality rabbit anti-human immune-related gene guanosine triphosphate (IRGM) polyclonal antibody.
研究目的：克隆表达人肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化。
Objective: To clone and express the gene of human enterokinase light chain which would be used in the cleavage and purification of fusion proteins.
目的构建凋亡素原核表达系统，以制备抗原物质凋亡素融合蛋白。
Objective to construct an apoptin expression system to produce an antigen, apoptin fusion protein.
结果：成功克隆了CAT的基因片段，并在大肠杆菌中得到其融合蛋白的表达。
Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.
首先以非融合蛋白形式进行表达。
这项实验发现大鼠的血液干细胞与小鼠的Purkinjie神经元相融合后，停止表达血细胞蛋白，转而表达大鼠神经元特异性基因产物。
This showed nuclei from rat blood stem cells that had fused to Purkinje cells in mice stop expressing blood cell proteins and begin to express rat neuron-specific gene products.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
结果：将编码人单核细胞趋化蛋白—1（MCP—1）基因克隆至融合表达载体pGEX—2T中，DNA测序证实正确。
Results: A gene fragement encoding MCP-1 was cloned into the fusional expression vector PGEX-2T. DNA sequencing indicated that it was correct.
结论：利用杆状病毒表达系统，成功地表达同时具有核蛋白及糖蛋白g 2生物学活性的融合蛋白g2s0 . 7，为进一步研究其免疫学特性奠定了基础。
CONCLUSION: the successful expression of fusion protein G2S0.7 with biological activity in insect cells, which lays the foundation for further research on its immunological characteristic.
结果：经表达和纯化获得了纯度达90%以上的融合蛋白；
Results: The recombinant proteins were expressed successfully and the purity was over 90% after purified by affinity chromatography.
结论成功克隆人胰腺组织激肽原酶基因，并高效表达融合蛋白，为进一步开发基因工程药物打下基础。
CONCLUSION the fusion protein of the cloned kininogenase gene was highly expressed in E. coli and could be used for the development of its biological products.
将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上，构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
结果：融合蛋白以可溶性形式存在于细菌裂解液的上清中，其表达量为菌体总蛋白量的7%。
RESULTS: Fusion protein existed in supernatant of the bacteria lysate and its expression level was about 7% of the total bacteria protein.
结论：成功克隆葡糖基转移酶CAT基因并获得其融合蛋白的表达，为后续研究奠定了基础。
Conclusion: the target gene and its fusion protein was successfully expressed, which provide a base for the further research.
如果大肠杆菌中表达的VP1和VP2融合蛋白也能形成中和抗原表位，则解决了这个问题。
If the fusion protein of VP1 and VP2 that was expressed in E. coli can form neutralizing antigen epitopes, this problem is resolved.
结论：用硫氧还蛋白融合表达系统在大肠杆菌中表达的小鼠内皮抑素重组融合蛋白易纯化并具有高活性。
CONCLUSION: The mouse endostatin recombinant fusion protein expressed in E. coli by using thioredoxin fusion expression system is easy to be purified and possesses high activity.
该融合蛋白的成功表达为猪传染性胸膜肺炎亚单位疫苗的研制奠定了基础。
The fusion protein has been proved having immunogenicity when detected by Western blot, which demonstrates that it may be useful in the development of porcine pleuropneumonia subunit vaccine.
该融合蛋白的成功表达为猪传染性胸膜肺炎亚单位疫苗的研制奠定了基础。
The fusion protein has been proved having immunogenicity when detected by Western blot, which demonstrates that it may be useful in the development of porcine pleuropneumonia subunit vaccine.
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