将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上,构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
结果从活化的人白细胞中克隆到IDO基因编码区全长,并构建了IDOEGFP融合蛋白表达载体。
Results The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed.
将其与GUS报告基因融合在一起,构建了植物表达载体,并由农杆菌介导法导入水稻品种‘中花11’中。
It was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into Zhonghua 11, a rice variety, by Agrobacterium meditation.
结果:将编码人单核细胞趋化蛋白—1(MCP—1)基因克隆至融合表达载体pGEX—2T中,DNA测序证实正确。
Results: A gene fragement encoding MCP-1 was cloned into the fusional expression vector PGEX-2T. DNA sequencing indicated that it was correct.
目的构建克隆载体,分析隐匿性乙型肝炎病毒S基因的突变情况,构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
本实验以轮状病毒的VP7为目的基因,构建了VP7基因的表达载体和VP7-CTB融合表达载体;
In this study, VP7 gene and CTB-VP7 fusion gene expression vectors were constructed, and a high-efficient genetic transfomation system of carrot(Daucus carota L. )
目的:获得RECK基因GFP融合蛋白表达载体并使其在脑胶质瘤细胞SHG44中表达。
Objective: To obtain GFP-RECK expression vector and to express it in glioma cell line SHG44.
目的:获得RECK基因GFP融合蛋白表达载体并使其在脑胶质瘤细胞SHG44中表达。
Objective: To obtain GFP-RECK expression vector and to express it in glioma cell line SHG44.
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