本文介绍了与鱼类种质鉴定相关的一种新的鉴定方法———养殖鱼类血清转铁蛋白鉴定法。
A new identifying method relating with fish germplasm identifying, the serum transferrin identifying method of breeding fishes was introduced.
用免疫共沉淀的方法鉴定E4B 的相互作用蛋白质。
Co-immunoprecipitation was used to identify the interactive protein.
一个令人振奋的发现：在中东家庭里发现的大多数遗传缺陷并不在基因的商业部分里——鉴定大脑蛋白质的密码。
One encouraging finding: most of the genetic defects identified in the Middle Eastern families were not in the business part of the gene -the part that codes for a critical brain protein.
Craig的团队鉴定的这种蛋白可以通过去除并分解肿瘤细胞表面的一种侵袭性酶而抑制肿瘤细胞的扩散。
The regulatory protein identified by Dr Craig's team inhibits the spread of cancer cells by removing and breaking down an invasive enzyme on the surface of cancer cells.
随后，研究者就将被捕获的蛋白质分离出来，并进行鉴定。
The researchers then separated out the captured proteins and identified them.
分离、鉴定与生长素结合的蛋白是生长素受体研究中的一个重要方面。
Isolation and identification the auxin binding protein is one of the major works in the studying of auxin receptor.
通过蛋白质组学方法对有或无某种疾病的人群蛋白表达的比较，鉴定出与该疾病特异相关的独特蛋白表达谱或表达模式。
Proteomic methods compare protein expression in patients with and without a gien condition to identify unique patterns or profiles of protein expression related specifically to that condition.
采用多维色谱-串联质谱联用技术分析鉴定小鼠肝脏质膜蛋白质。
Multidimensional liquid chromatography coupled with tandem mass spectrometry was used to identify mouse liver plasma membrane(PM) proteins.
方法：用微柱凝胶法对693例标本进行不规则抗体筛选，用酶、抗球蛋白法进行鉴定，并观察不规则抗体检出的阳性率。
Methods: Employed irregular antibody screening on 693 samples with microcolumn gel agglutination assay, and identified with enzyme and antiglobulin method to observe the positive rate.
另一项研究中，研究人员鉴定1型糖尿病青少年小瞳孔大小同12年后微白蛋白尿及视网膜病之间的关系。
In a separate study, researchers identified a predictive relationship between small pupil size in adolescents with type 1 diabetes and the presence of microalbuminuria and retinopathy 12 years later.
这就使得用小麦水溶性蛋白作为品种鉴定和遗传纯度分析成为可能。
This made wheat identification and genetic purity analysis by using water soluble proteins possible.
双相聚苯稀酰氨凝胶电泳（2DPAGE）与质谱（MS）是分辨和鉴定蛋白的最常用的方法，但也存在有缺点。
Two dimensional polyacrylamide gel electrophoresis(2D PAGE) followed by mass spectrometry(MS) is the most widely used method of protein resolution and identification. But it exist shortcomings.
质谱鉴定有差异表达的其中4个蛋白点。
The 4 differentially expressed protein spots were identified by mass spectrometry.
应用免疫过氧化酶技术鉴定甲状腺球蛋白和甲状腺素在甲状腺组织中的分布，可以了解甲状腺滤泡细胞的功能状态。
Employing immunoperoxidase technique to determine the distribution of thyroglobulin and thyroxine in the thyroid made us possible to understand the functional state of follicular cell.
这是鉴定某些蛋白质的一种极好的方法。
This is an excellent method for identification of certain proteins.
在对差异点进行的质谱鉴定中发现了热休克蛋白27在肿瘤中表达下调。
Heat shock 27 was the most interesting differential expression protein and was down expression in papillary thyroid cancer.
结果(1)培养的细胞经形态学观察和波形蛋白sp染色，鉴定为成纤维细胞。
Results (1) the cultured cells identified by morphology and SP staining with vimentin showed the characteristic of fibroblasts.
传统的亚细胞分离技术结合质谱鉴定技术为亚细胞蛋白质组学的研究提供了技术平台。
The classical subcellular fractionation methods coupled with mass spectrometry identification technique have created opportunities for the study of subcellular fractions.
目的应用蛋白质质谱分析方法寻找胃癌鉴定的生物标志物。
Objective to search for the biomarker used to determine gastric cancer by the application of protein mass spectrometry analysis.
用十二烷基硫酸钠-凝胶电泳(SDS -PAGE)和二维凝胶电泳鉴定蛋白质纯度。
The obtained proteins were checked for the purity through SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
指出这些质谱新技术已被证明在多肽和蛋白质的鉴定、单核苷酸、核酸、糖蛋白的分析等领域是很有效的工具。
These new techniques have been proven to be the powerful tool for identifying protein, polypeptide, and analyzing single nucleotide polymorphism, nucleic acids, glycoprotein, etc.
结论：应用“四步提取法”使得酶解肽段提取率和蛋白质的有效鉴定率明显提高。
Conclusion: the application of "four-step extraction" method enhances the peptide recovery during in-gel digestion, and increases identification of the protein.
生物信息学是蛋白质组研究不可缺少的技术，通过比较质谱分析结果与蛋白质数据库中的氨基酸序列可实现蛋白质的鉴定。
Bioinformatics which is an indispensable technique can evaluate the protein by comparing the results of mass spectrometry and the amino acid rank in protein databank.
肽质谱指纹图分析是一种常用的蛋白质的鉴定方法。
Peptide mass fingerprinting analysis of proteins is the most widely used method for protein identification.
方法：以TRF1抗体应用免疫共沉淀方法，从细胞蛋白抽提物中分离TRF1蛋白复合物，并作蛋白质肽指纹谱鉴定；
Methods: The co immunoprecipitation assay was employed to isolate TRF1 protein complex and the immunoprecipitate was subjected to MALDI TOF mass spectrometry for protein identification.
本培养法在细菌蛋白质产物分析及临床细菌鉴定中是很有实用价值的。
The data indicate that this cultural method may be useful in analyzation of bacterial protein products and clinical identification of bacteria.
本培养法在细菌蛋白质产物分析及临床细菌鉴定中是很有实用价值的。
The data indicate that this cultural method may be useful in analyzation of bacterial protein products and clinical identification of bacteria.
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