• 方法采集33例正常人26牙周炎患者全唾液,运用聚丙烯酰胺凝胶聚焦方法观察其蛋白质等电点分布。

    Methods: Saliva was collected from 33 healthy and 26 periodontitis individuals respectively, and analysed with isoelectric focusing in polyacrylamide gels.

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  • 通过对双向图谱初步观察可以看到蛋白质主要集中等电点5—8分子量28kd—66 KD之间

    From 2-d electrophoresis maps, we can find that most of proteins focused between pI5 - pI 8 and molecular weight 28kd-66kd.

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  • 并测定葡萄籽蛋白质等电点PI)为3.8。

    The PI of the protein is 3.8.

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  • 蛋白质首先毛细管聚焦过程根据它们等电差异得到分离然后喷雾质谱鉴定。

    Proteins were separated and resolved in CIEF according to their differences in isoelectric point (pI), and then characterized by ESI MS.

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  • 发现两者蛋白质组成分子量等电氨基酸组成上颇为相近。免疫扩散试验证实两者免疫交叉反应

    Some similarities in polypeptide components, molecular weight, isoelectric point and amino acid composition were found, but no immunocross reaction between them was observed.

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  • 发现两者蛋白质组成分子量等电氨基酸组成上颇为相近。免疫扩散试验证实两者免疫交叉反应

    Some similarities in polypeptide components, molecular weight, isoelectric point and amino acid composition were found, but no immunocross reaction between them was observed.

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