方法:采集33例正常人,26例牙周炎患者全唾液,运用聚丙烯酰胺凝胶等电聚焦方法观察其蛋白质等电点分布。
Methods: Saliva was collected from 33 healthy and 26 periodontitis individuals respectively, and analysed with isoelectric focusing in polyacrylamide gels.
通过对双向电泳图谱的初步观察可以看到,蛋白质主要集中在等电点5—8和分子量28kd—66 KD之间。
From 2-d electrophoresis maps, we can find that most of proteins focused between pI5 - pI 8 and molecular weight 28kd-66kd.
并测定了葡萄籽蛋白质的等电点(PI)为3.8。
蛋白质首先在毛细管等电聚焦过程中根据它们等电点的差异得到分离,然后被电喷雾质谱鉴定。
Proteins were separated and resolved in CIEF according to their differences in isoelectric point (pI), and then characterized by ESI MS.
发现两者在蛋白质组成,分子量,等电点及氨基酸组成上颇为相近。但免疫扩散试验证实两者无免疫交叉反应。
Some similarities in polypeptide components, molecular weight, isoelectric point and amino acid composition were found, but no immunocross reaction between them was observed.
发现两者在蛋白质组成,分子量,等电点及氨基酸组成上颇为相近。但免疫扩散试验证实两者无免疫交叉反应。
Some similarities in polypeptide components, molecular weight, isoelectric point and amino acid composition were found, but no immunocross reaction between them was observed.
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