用免疫共沉淀的方法鉴定E4B 的相互作用蛋白质。
Co-immunoprecipitation was used to identify the interactive protein.
对于蛋白质的结晶，沉淀剂主要在溶剂上产生作用。
For protein crystallization, the main impact of precipitant is on the solvent.
聚乙二醇6000（PEG）是一种水溶性非离子聚合物，可使蛋白质发生沉淀作用，并且其对被沉淀的蛋白质有较好的选择性；
Polyethylene glycol 6000(PEG) is a kind of water soluble and non-ionic polymer. lt can make protein to precipitate and has a better alternative to precipitation of protein.
目的：评价共焦点显微镜对亲水性软性角膜接触镜片上蛋白质沉淀情况的观察作用。
Objective: To evaluate the value of the confocal microscope in observing protein deposits on the hydrophilic contact lens.
在HEK293T细胞中，通过双荧光报告系统检测E4B对TLR信号通路的影响，并且用免疫共沉淀的方法鉴定E4B的相互作用蛋白质。
In HEK293T, Dual-Luciferase Reporter Assay System was used to evaluate the effect of E4B on TLR pathway, and co-immunoprecipitation was used to identify interactive protein.
在HEK293T细胞中，通过双荧光报告系统检测E4B对TLR信号通路的影响，并且用免疫共沉淀的方法鉴定E4B的相互作用蛋白质。
In HEK293T, Dual-Luciferase Reporter Assay System was used to evaluate the effect of E4B on TLR pathway, and co-immunoprecipitation was used to identify interactive protein.
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