将纯化的在大肠杆菌中表达的鹦鹉热衣原体（Cps）的重组主要外膜蛋白（MOMP）与油佐剂混合。
The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci (Cps) expressed in E. coli was mixed with oil adjuvant.
表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
利用鹦鹉热衣原体重组主要外膜蛋白（MOMP）在大肠杆菌中的表达产物，纯化后与白油佐剂乳化成疫苗。
The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci(Cps)expressed in E. coli was mixed with oil adjuvant and manufactured as MOMP-vaccine.
研究目的：克隆表达人肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化。
Objective: To clone and express the gene of human enterokinase light chain which would be used in the cleavage and purification of fusion proteins.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
首先，我们在第11章中知道了关于大肠杆菌表达的蛋白产物的纯化工艺优化和特性研究。
First, we learn in Chapter 11 about process optimization and characterization studies for the purification of an E. coli-expressed protein product.
目的：合成胆汁三烯结合蛋白(BBP)基因并在大肠杆菌中表达，获得重组BBP纯化制品。
Objective: To synthesize bilin binding protein (BBP) gene sequence, express BBP efficiently in Escherichia coli and purify the recombinant protein.
完成了NKG2D的原核表达载体的构建，表达并纯化了重组NKG2D蛋白。
The NKG2D prokaryotic expression vector was successfully constructed. The recombinant NKG2D is expressed and purified.
目的：表达和纯化半乳糖凝集素- 1融合蛋白。
Objective: To express and purify the fusion protein of galectin-1.
介绍了近年来重组蛋白质的常用表达系统以及各种分离纯化技术等研究和应用的进展情况。
The study and application were introduced on the expression system in common use and separation and purification technique of recombinant proteins in recent years.
获取人蛋白酪氨酸磷酸酯酶4A2（PTP4A2）基因并高效表达纯化，对于产物的体外活性进行测定。
To obtain human protein tyrosine phosphatase type 4A2 (PTP4A2) gene expressed in E. coli and purify the target proteins.
通过基因克隆等方法，选择原核表达系统，并对目的蛋白进行分离纯化，初步获得有活性的人内毒素结合肽ebp。
We isolated and purified EBP effectively, and obtained EBP with biological activity for the first time with gene cloning method and prokaryotic expression system.
目的克隆和表达日本血吸虫钙磷蛋白基因，纯化表达产物。
Objective To clone and express Schistosoma japonicum (Sj) calcyphosine gene, and purify the expressed protein.
结论：用硫氧还蛋白融合表达系统在大肠杆菌中表达的小鼠内皮抑素重组融合蛋白易纯化并具有高活性。
CONCLUSION: The mouse endostatin recombinant fusion protein expressed in E. coli by using thioredoxin fusion expression system is easy to be purified and possesses high activity.
目的克隆人热休克蛋白72 (HSP72)基因，原核表达并纯化蛋白产物，以探讨其生物学功能。
AIM To clone human heat shock protein 72 (HSP72) gene, do prokaryotic expression and purify HSP72 protein for further study.
目的构建人细胞色素P450 4f 2基因野生型、V81G和V433 M突变型表达载体，在大肠杆菌中表达并纯化出CYP4F2蛋白。
Objective to construct the expression vectors of human cytochrome P450 (CYP) 4f2 wild type, V81G and V433M Variants and express and purify CYP4F2 protein in e.
简要介绍了去垢剂在膜蛋白研究中的最新应用进展，步及去垢剂在膜蛋白离体表达、分离和纯化、以及结构研究中的应用。
In this article, the latest bibliography were reviewed related to applications of detergents to in vitro expression, purification, and structural investigation of membrane proteins.
原核表达并纯化单纯疱疹病毒特异性糖蛋白抗原，用于建立单纯疱疹病毒的临床检测方法。
To express and purify the specific glycoprotein antigens of herpes simplex virus (HSV) as used in HSV clinical detection.
目的：克隆长双歧杆菌ncc 2705株果糖结合蛋白bl 0033的基因，利用大肠杆菌表达GST -BL 0033融合蛋白并纯化。
Objective: to clone the gene of fructose binding protein BL0033 from Bifidobacterium longum NCC2705, and express and purify fusion protein GST-BL0033 in e.
这一方法可能是解决植物肌动蛋白及其他在包涵体中表达蛋白难以正确折叠和大量纯化的有效途径之一。
This may be a good method for obtaining soluble and abundant plant actin isoforms and other proteins which could not fold exactly and exist in inclusion body by prokaryotic expression.
目的将肌腱蛋白-R不同功能片段在原核中表达、纯化，并研究其初步的生物学功能。
AIM To express and to purify recombinant tenascin R domains and to study their functions.
结论：构建了8r - MUC1核心肽融合蛋白原核表达载体并成功表达与纯化出具有生物学活性的融合蛋白。
Conclusion the prokaryotic expression vector of 8r-muc1 core peptide fusion protein has been constructed, and the fusion protein with biological activity has been successfully expressed and purified.
目的构建结核杆菌分泌蛋白mpt53原核表达载体并进行表达和纯化。
Objective to construct a expression vector of MPT53 of mycobacterium tuberculosis and identify and purify the protein in the e.
SDS电泳分析结果表明EPSPS重组蛋白的表达量在55%以上，纯化的EPSPS重组蛋白纯度可以达到90%。
The SDS gel electrophoresis analysis shows that the expression of EPSPS recombined protein is over 55% and the purified EPSPS recombined protein reached as 90%.
新基因进行原核表达并纯化蛋白。
结果：经表达和纯化获得了纯度达90%以上的融合蛋白；
Results: The recombinant proteins were expressed successfully and the purity was over 90% after purified by affinity chromatography.
目的构建人高迁移率族蛋白1（HMGB1）编码基因的表达载体，获得纯化的重组蛋白，研究其生物学功能。
Objective To construct the recombinant vector, the expression of human HMGB1 code gene and to study its function.
目的构建天花粉蛋白(TCS)突变体基因，并进行表达及纯化。
Objective To construct and express a trichosanthin (TCS) gene mutant and purify the expressed product.
目的构建天花粉蛋白(TCS)突变体基因，并进行表达及纯化。
Objective To construct and express a trichosanthin (TCS) gene mutant and purify the expressed product.
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