逆转录多聚酶链反应检测间质胶原酶及金属蛋白酶组织抑制因子- 1基因表达水平。
The gene expression levels for interstitial collagenase and tissue inhibitor of metallo-proteinase-1 (TIMP-1) were measured by reverse transcription polymerase chain reaction.
应用免疫组化s - P法检测24对食管癌组织及相应正常食管组织中抑癌基因pten的蛋白质表达水平。
Expression levels of PTEN gene were detected by the SP immunohistochemical technique in the selected 24 pairs of tumor tissues and the related normal tissues.
结论：FCM是检测膀胱癌多药耐受蛋白质表达水平和MRP功能水平的好方法，DNR是FCM检测MRP功能的良好探针。
Conclusions: FCM is a good method to detect MDR protein and MRP function of bladder carcinoma, and DNR is a good probe to detect MRP function by FCM.
方法：应用基因芯片、反转录PCR（RT-PCR）、流式细胞术等方法从分子和蛋白水平检测两种细胞系中CXCR4的表达情况。
Methods: Gene chip and RT-PCR were used to study expression of CXCR4 mRNA in two cell lines and the CXCR4 surface expression was also examined by flow cytometry analysis.
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
本专利提 出了一种检测转录因子表达和活化水平的新方法“核酸外切酶III消化微孔板包被特殊标记双链核酸分子检测转录因子蛋白”。
The present invention proposes exonuclease III digesting double stranded nucleic acid molecule coated in microporous plate and containing special marker to detect transcription factor protein.
将表达的融合蛋白作为包被抗原，建立了间接酶联免疫吸附试验(间接elisa)的诊断方法以检测CDV血清抗体水平。
The method of indirect ELISA to detect CDV antibody in blood serum was established, using recombinant nucleocapsid protein as envelope antigen.
目的观察脑红蛋白（NGB）在足月健康新生儿和宫内窘迫新生儿血清中的水平，探讨宫内缺氧对血清NGB表达的影响及NGB作为早期判断宫内缺氧检测指标的价值。
Objective To observe the change of serum neuroglobin(NGB)of normal full-term and fetal distress infants, and to explore the sensitivity and validity of NGB as potential biomarker for brain injury.
方法用免疫组织化学方法检测80例食管癌及其相应的手术远端正常食管组织中pten蛋白的表达水平。结果PTEN在食管鳞癌中的表达率明显低于癌旁正常食管黏膜组织P。
Methods Expression of PTEN was examined by immunohistochemical SP method in 80 cases of esophageal carcinoma and normal esophageal tissues closely adjacent to carcinoma.
分别以重组蛋白疫苗、商品疫苗和PBS免疫仔猪，优化检测免疫抗体水平的间接ELISA条件，通过比较三个免疫组的抗体水平来评价表达蛋白作为免疫抗原的有效性。
Piglets of each groups were vaccinated respectively with recombinant protein vaccine, commodity vaccine and PBS as contrast. The serum IgG anti-protein of each groups were detected by indirect ELISA.
同时通过检测胃癌组织中基因的蛋白表达水平，了解基因高甲基化与其蛋白表达缺失之间的关系。
The expression of p16 and DAPK protein were detected to analyse the relationship between the promoter hypermethylation and the loss of protein expression.
同时通过检测胃癌组织中基因的蛋白表达水平，了解基因高甲基化与其蛋白表达缺失之间的关系。
The expression of p16 and DAPK protein were detected to analyse the relationship between the promoter hypermethylation and the loss of protein expression.
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