首先，我们在第11章中知道了关于大肠杆菌表达的蛋白产物的纯化工艺优化和特性研究。
First, we learn in Chapter 11 about process optimization and characterization studies for the purification of an E. coli-expressed protein product.
目的：表达和纯化半乳糖凝集素- 1融合蛋白。
Objective: To express and purify the fusion protein of galectin-1.
介绍了近年来重组蛋白质的常用表达系统以及各种分离纯化技术等研究和应用的进展情况。
The study and application were introduced on the expression system in common use and separation and purification technique of recombinant proteins in recent years.
目的构建人细胞色素P450 4f 2基因野生型、V81G和V433 M突变型表达载体，在大肠杆菌中表达并纯化出CYP4F2蛋白。
Objective to construct the expression vectors of human cytochrome P450 (CYP) 4f2 wild type, V81G and V433M Variants and express and purify CYP4F2 protein in e.
目的克隆和表达日本血吸虫钙磷蛋白基因，纯化表达产物。
Objective To clone and express Schistosoma japonicum (Sj) calcyphosine gene, and purify the expressed protein.
简要介绍了去垢剂在膜蛋白研究中的最新应用进展，步及去垢剂在膜蛋白离体表达、分离和纯化、以及结构研究中的应用。
In this article, the latest bibliography were reviewed related to applications of detergents to in vitro expression, purification, and structural investigation of membrane proteins.
这一方法可能是解决植物肌动蛋白及其他在包涵体中表达蛋白难以正确折叠和大量纯化的有效途径之一。
This may be a good method for obtaining soluble and abundant plant actin isoforms and other proteins which could not fold exactly and exist in inclusion body by prokaryotic expression.
本试验对表达的杀白细胞素f和S双组分蛋白进行纯化，制备成免疫原免疫家兔。
In this experiment, the expressed LukF and LukS proteins were purified and were prepared separately as immunogens for rabbit immunization.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
目的构建结核杆菌分泌蛋白mpt53原核表达载体并进行表达和纯化。
Objective to construct a expression vector of MPT53 of mycobacterium tuberculosis and identify and purify the protein in the e.
结果：经表达和纯化获得了纯度达90%以上的融合蛋白；
Results: The recombinant proteins were expressed successfully and the purity was over 90% after purified by affinity chromatography.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
目的：构建融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白。
AIM: to construct the recombinant expression vector and to obtain large amount of mouse Cyclin D1 protein fused to PTD.
目的：构建融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白。
AIM: to construct the recombinant expression vector and to obtain large amount of mouse Cyclin D1 protein fused to PTD.
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