这将为进一步研究其体外可控性剪接、构建高效的蛋白纯化系统和深入研究蛋白质内含子的剪接机制提供基础。
This will help us to investigate controllable protein cleavage in vitro, further establish efficient protein purification system and investigate splicing mechanism of split-inteins.
介绍了近年来重组蛋白质的常用表达系统以及各种分离纯化技术等研究和应用的进展情况。
The study and application were introduced on the expression system in common use and separation and purification technique of recombinant proteins in recent years.
结合物经快速蛋白质液相层析系统用凝胶色谱柱纯化后，用SDS PAGE鉴定各收集峰。
Every collected peak was identified by SDS-PAGE after purified with gel chromatography by fast protein liquid chromatography system.
通过基因克隆等方法，选择原核表达系统，并对目的蛋白进行分离纯化，初步获得有活性的人内毒素结合肽ebp。
We isolated and purified EBP effectively, and obtained EBP with biological activity for the first time with gene cloning method and prokaryotic expression system.
结论：用硫氧还蛋白融合表达系统在大肠杆菌中表达的小鼠内皮抑素重组融合蛋白易纯化并具有高活性。
CONCLUSION: The mouse endostatin recombinant fusion protein expressed in E. coli by using thioredoxin fusion expression system is easy to be purified and possesses high activity.
结论GST-3C蛋白酶不仅可以应用于融合蛋白的酶切，而且为引入3C蛋白酶识别位点的串联亲和纯化（TAP）系统提供又一种酶切工具。
Conclusion The recombinant GST-3C protease can be not only applied to the cleavage of fusion proteins, but also be used as a tool-enzyme in TAP system containing 3C recognition sequence.
目的建立HPV16 L 1蛋白原核表达系统的纯化方法，纯化目的蛋白。
Objective To establish a method of purification of HPV16L1 protein expressed in a prokaryotic system and obtain the purified protein.
结果HPV16L1蛋白在原核表达系统以不溶性包涵体形式存在，通过本方法可以获得纯化的蛋白。
Results HPV16L1 proteins formed inclusion bodies in bacterial expression system, this assay could purified HPV16L1 proteins.
结果HPV16L1蛋白在原核表达系统以不溶性包涵体形式存在，通过本方法可以获得纯化的蛋白。
Results HPV16L1 proteins formed inclusion bodies in bacterial expression system, this assay could purified HPV16L1 proteins.
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