摸索建立了肝组织蛋白样品的制备方法，并初步优化了蛋白质组学研究中的实验条件。
To explore the establishment of protein sample preparation methods for liver tissue and optimize the experimental Parameters.
包括细胞总蛋白样品的制备、微珠上抗原-抗体反应及微珠洗涤条件、进样方式的选择与改进。
This part includes preparation of total protein samples from the culture cells, on-bead immuno-binding reaction and wash step, and choice of sampling.
该技术平台的构建涉及到蛋白样品的制备、封闭液的选择、点样条件的选择、抗体的浓度及其杂交时间及数据的处理等多个方面。
This technology platform construction involves protein samples preparation, the choice of sealed liquid, the conditions of printing, concentration and hybrid time of antibody and data processing, etc.
过去甲基化的结构研究被阻碍的原因是因为很难制备组蛋白特定部位赖氨酸甲基化的均一样品。
Past structural studies of methylation were hindered by the extreme difficulty of constructing homogeneous samples of histones bearing methyl groups attached to specific lysine residues.
在所有样品中，线粒体呼吸链蛋白细胞色素氧化酶没有变化， 表明线粒体的制备方法是可靠的。
In all those samples, there were no alterations in the subcellular distribution of cytochrome oxidase, a mitochondrial respiratory chain protein.
最后我们将优化的蛋白质组学样品制备方法应用于鸡的脾脏和胸腺，也获得了高质量的二维电泳图谱。
In addition, this optimized protocol was also successfully applied in 2-DE analysis of spleen and thymus in chicken.
最后我们将优化的蛋白质组学样品制备方法应用于鸡的脾脏和胸腺，也获得了高质量的二维电泳图谱。
In addition, this optimized protocol was also successfully applied in 2-DE analysis of spleen and thymus in chicken.
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