连接产物转化至BL21感受态细胞，涂板、挑选单克隆进行菌落PCR。

The products were transformed into BL21 competent cells, coated plates, select monoclonal colony PCR.

youdao

连接产物转化至BL21感受态细胞，涂板、挑选单克隆进行菌落PCR。

The products were transformed into BL21 competent cells, coated plates, select monoclonal colony PCR.

youdao