目的建立应用任意引物聚合酶链反应进行真菌菌型鉴定的方法。
Objective To establish the method to identify fungus using the arbitrarily primed polymerase chain reaction (APPCR).
方法取肺外结核病灶中的坏死物质作分枝杆菌培养、菌型鉴定及药物敏感性试验。
Method The mycobacterium culture, appraising of bacterial type and medicine sensitiveness test of focal necrosis material of outer-pulmonary tuberculosis are completed.
目的:建立梭菌属神经毒素基因快速鉴定和分型的PCR方法。
Objective: to establish a PCR method for gene identification and genotyping of clostridial neurotoxin.
目的研究红色毛癣菌dna分型并探讨DNA分型技术在临床菌种鉴定以及流行病学中的应用。
Objective to investigate the DNA type of Trichophyton rubrum and to discuss the application of DNA typing method in its clinical identification and epidemiology study.
目的从分子水平鉴定四川省2005年不明原因疾病病原菌的属、种、型及毒力基因,为诊断和治疗提供科学依据。
Objective to identify the genus, species, type and toxic gene of pathogenic bacteria causing disease outbreak in Sichuan in 2005, in order to provide scientific basis for diagnosis and treatment.
方法采用系统生化和血清学方法鉴定菌群和血清型,采用K - B法检测菌株的耐药性。
Method Shigella bacterial flora and serotype were identified by systematic biochemistry and serological method. Drug resistance was detected by K-B.
结果对25株发酵型革兰氏阴性杆菌鉴定符合率为100%,对150株志贺氏菌鉴定符合率为99.3%。
Results showed that the rate of accuracy was 100% in 25 symogenous Gram-negative bacteria lines and 99.3% in 150 Shigella lines.
目的用PCR方法扩增红色毛癣菌和须癣毛癣菌临床分离株的DNA ,观察PCR指纹的差异以及基因型与传统表型的关系,建立一种红色毛癣菌分子生物学鉴定方法。
Objective To observe the unique DNA profile and the relationship between DNA profile and phenotype of Trichophyton rubrum , and establish an effective molecular method to identify T. rubrum .
应用MALDI-TOF分析方法可实现在细菌属、种、菌株和血清型水平上对食品病原菌快速、准确地区分和鉴定,显示出广泛的应用前景。
MALDI-TOF mass spectrometry can be used for rapid, accurate and sensitive discrimination and identication of food-borne pathogens, showing the useful significances.
应用MALDI-TOF分析方法可实现在细菌属、种、菌株和血清型水平上对食品病原菌快速、准确地区分和鉴定,显示出广泛的应用前景。
MALDI-TOF mass spectrometry can be used for rapid, accurate and sensitive discrimination and identication of food-borne pathogens, showing the useful significances.
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