表达的融合蛋白约占菌体蛋白总量的2 8%。
食品工业废渣以发酵技术生产菌体蛋白饲料的研究。
Single cell protein feedstuff production from food industry wastes with fermentation technology.
其中菌体蛋白、肽类、游离氨基酸含量高，营养成分吸收率高。
Content of thalline protein, peptide and free amino acid of the forage is high, and nutrient composition absorptivity is high.
此方法可以用于重组溶葡萄球菌酶中残余宿主菌菌体蛋白含量的测定。
The method is simple, sensitive and accurate, so the trace of bacteria protein in recombinant lysostaphin products can be detected.
当细菌遭遇消毒剂后，Hsp33立即采取行动以保护细菌体蛋白抵抗漂白剂的诱导聚集作用。
When bacteria encounter the disinfectant, Hsp33 jumps into action to protect bacterial proteins against bleach-induced aggregation.
结果表明：和对照组相比，酶解血粉显著提高了瘤胃的肽含量和菌体蛋白的产量（P<0.05）；
The results showed that content of peptide and yield of MCP were significantly increased by enzymolysis blood meal(P<0.05).
以味精生产中的废弃物——谷氨酸菌体蛋白为原料，经过稀酸预处理后，用蛋白酶水解获得复合氨基酸水解液。
Glu thalli in the production of MSG, having been treated with thin acid, was hydrolyzed by protease to obtain amino acid hydrolysate.
对于含有多种成分且各蛋白质成分的分子量相差较小的菌体蛋白质的提纯，用一般的沉淀法、层析法均达不到理想效果。
Objective:To purify bacterium protein made up of many compositions whose molecular weights are little difference. Methods:results of using sediment and layer-division are not satisfying.
菌体经过超声破碎后反复洗涤包涵体，融合蛋白纯度达到70%，进一步用离子交换层析和凝胶过滤层析纯化使其纯度达95%以上。
The purity of fusion protein was about 70% after ultrasonic decomposition and washing repeatedly, and reached above 95 % after purification by ion exchange and gel-filtration chromatography.
用谷胱甘肽琼脂糖凝胶4b亲和介质从菌体裂解液中纯化了GST -MBD4融合蛋白。
The GST-MBD4 fusion protein was purified from cell lysates using glutathione Sepharose 4b affinity chromatography.
结果：融合蛋白以可溶性形式存在于细菌裂解液的上清中，其表达量为菌体总蛋白量的7%。
RESULTS: Fusion protein existed in supernatant of the bacteria lysate and its expression level was about 7% of the total bacteria protein.
以此纯化蛋白为靶，采用固体包被法对噬菌体展示随机十二肽库及环七肽库进行亲和筛选。
The purified protein coated on polystyrene plate was used as the target to carry out four rounds of bio-panning.
PP 65目的蛋白以融合蛋白的形式表达在T7噬菌体的头蛋白部位，每个噬菌体颗粒表面可表达5 ~15个拷贝。
The PP65 protein was expressed on the surface of the T7 as head fusion protein. There were 5 to 15 copies on the each phage.
目的应用噬菌体表面展示技术寻找原因不明性肝炎血清蛋白成分在肝细胞表面的结合蛋白，探讨原因不明性肝炎发病机制。
To search for liver surface binding protein of serum protein composition in idiopathic hepatitis by phage display technique, and investigate the pathogenesis of idiopathic hepatitis.
结果经SDSPAGE和薄层扫描分析表明，外源蛋白表达量占菌体裂解蛋白总量的6 . 4 %。
Results SDS PAGE and thin layer scanning showed that the expressed product contained about 6.4% of total somatic protein.
结果：SDS - PAGE和薄层扫描分析表明外源蛋白的表达量占菌体裂解蛋白总量的18.6%。
Analysis of SDS-PAGE and thin layer scanning results showed that amount of expressed fusion protein was 18.6% in total lysis protein of bacteria.
噬菌体表面展示技术广泛应用于疾病诊断、特异性抗体及蛋白药物生产等。
The phage surface display technology is widely used in disease diagnosis, specific antibody, and protein medicine production.
采用正常人和白血病患者的白细胞对人源噬菌体抗体库进行淘选，以获得对两种细胞表面蛋白特异的抗体。
A large human phage antibody library was subjected for panning with leukocytes from healthy donors and leukemia patients to select for specific antibodies against leukocytic surface proteins.
结论筛选的该噬菌体克隆展示肽可模拟JEVE蛋白的部分抗原性。
Conclusion This peptide displayed on positive phage may mimic partial antigenicity of JEV E protein.
结果融合蛋白的表达量占菌体总蛋白的20%。
Results the fusion protein expressed in e. coli account for 20% of the total bacterial protein.
利用聚金细菌体表存在一种特殊的蛋白质，对金原子进行代谢，聚集、吸附金原子，形成金结晶核。
A kind of special protein on the surface of agglutinative bacteria can be used to metabolize, collect and absorb the gold atoms, and then gold crystal nucleus will be formed.
噬菌体展示技术是一种有效的药物靶点筛选工具，可用其筛选中药的药物靶点蛋白。
The Phage display technology(PDT) is technology that expresses exogenic polypeptide with shell protein of phage by inserting the exogenic DNA into the shell gene.
同时还进行梯度实验分别对诱导的温度、时间和IPTG诱导时菌体浓度进行优化，并对蛋白的纯化方案进行摸索。
The induction temperature, induction time, and IPTG concentration were also optimized by a series of experiments. Further purification modes of this protein were also explored.
经薄层扫描分析，表达产物约占菌体总蛋白的29.8%。
Aanalysed with thin slice scan, the expression products occupy 29.8 % in the whole thallus proteins.
表达量约占菌体总蛋白量的21%。
结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体，为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research.
进而，采用流式细胞技术研究了不同蛋白酶体抑制剂以及TAP对噬菌体颗粒抗原交叉呈递的影响。
Furthermore, the effect of proteasome inhibitors and TAP on particle antigens cross presentation was investigated by FACS analysis.
类菌体四周的豆血红蛋白执行双重职能。
The leghaemoglobin surrounding the bacteroids serves a dual role.
棉籽水解蛋白可作为菌体发酵氮源。
Hydorlyzed cottonseed protein can be used as the nitrogen source of fermentation medium.
棉籽水解蛋白可作为菌体发酵氮源。
Hydorlyzed cottonseed protein can be used as the nitrogen source of fermentation medium.
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