本试验以甲型副伤寒沙门氏菌体抗原多克隆抗体的制备及提纯为例，提出可行的更简易的提纯方法。
In this experiment, we took the example of preparation and purification of polyclonal antibody of somatic antigen of Salmonella paratyphi a and found out a simpler feasible way of purification.
进而，采用流式细胞技术研究了不同蛋白酶体抑制剂以及TAP对噬菌体颗粒抗原交叉呈递的影响。
Furthermore, the effect of proteasome inhibitors and TAP on particle antigens cross presentation was investigated by FACS analysis.
目的：为了探讨噬菌体展示技术在筛选和鉴定病毒抗原表位研究中的应用前景。
Objective: to investigate the possibility of application of phage display technique in screening and identifying the virus antigenic epitopes.
目的以噬菌体颗粒抗原为模式抗原，研究其交叉呈递的可能机制。
Objective Phage particle antigens were used as a model antigen to investigate the potential mechanism of cross presentation.
目的从噬菌体肽库筛选日本血吸虫抗原模拟抗原表位。
Objective To screen the mimic antigen epitopes of Schistosoma japonicum from phage display peptide library.
结果筛选到的噬菌体能特异地与2F2结合，并且这种结合可被天然抗原所抑制。
RESULTS Short peptide displayed on phage specifically was bound to 2F2 and the binding was inhibited by natural JEV Ag.
用ELISA和流式细胞仪检测展示在噬菌体上CD 28的抗原性。
The antigenicity of CD28 displayed on phage was detected by ELISA and FCM.
以肺腺癌细胞系a2为抗原进行4轮筛选，得到富集的次级噬菌体表达文库。
After 4 rounds of screening with lung adenocarcinoma cell line A2 as antigen, an enriched secondary phage display library was obtained.
目的克隆白癜风患者淋巴细胞中原始配对的自身抗体基因，构建噬菌体抗体库并筛选针对黑素细胞膜抗原的特异性抗体。
AIM: To obtain in situ pairing of the variable region genes of the heavy and light chains of vitiligo patients' lymphocytes, then construct and screen a human ScFv phage antibody library.
结论筛选的该噬菌体克隆展示肽可模拟JEVE蛋白的部分抗原性。
Conclusion This peptide displayed on positive phage may mimic partial antigenicity of JEV E protein.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
目的：利用噬菌体展示肽库技术筛选鸡传染性支气管炎病毒的模拟抗原表位。
Objective:To obtain the mimotope of avian infectious bronchitis virus by phage display peptide library.
整个筛选过程中抗原与抗体的结合在液相中完成，不仅消除了固相介质对抗原表位的影响，也更有利于噬菌体抗体与抗原的充分作用。
The binding process of antigen and antibody completed in the solution, which doesn't have the shortage of conformational changes encountered in the immobilized screening.
以固相化的SARS抗原淘筛抗体库，并用ELISA检测噬菌体抗体结合SARS病毒的特异性。
Antibodies against SARS virus were screened by biopanning with immobilized virus antigen. The binding specificity of phage antibody to SARS virus was detected by ELISA.
目的构建人源抗红细胞A抗原单链噬菌体抗体库。
Objective To construct a phage display library of human single-chain Fv antibodies against blood group a substance.
利用展示有LKVIRK表位的杂合噬菌体作为抗原，免疫C57BL/6J小鼠，然后在小鼠的系统性念珠菌感染模型中评价其免疫保护作用。
The efficacy of hybrid phage particles displaying LKVIRK in inducing immune protection response were studies in C57BL/6J murine model of systemic C.
以该融合蛋白为固相抗原，对噬菌体抗体库进行3轮淘筛，并对所获阳性克隆进行抗原结合活性测定和DNA序列测定。
Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.
利用组合库和噬菌体展示技术，我们证明半抗原特异性抗体确实存在有外围载体的某些遗迹。
By using combinatorial library and phage display technologies on a hapten-specific antibody we were able to demonstrate that a peripheral carrier imprint indeed exists.
结论噬菌体展示技术完全可应用于病毒蛋白抗原序列的筛选，并具有简便、快速、准确的优点。
Conclusion the phage display technique can be applied to study the viral antigenic peptides with the advantages of simple, accuracy and rapidity.
这一属的有机体在抗原特性、糖发酵模式和噬菌体易感性的基础上被分离。
Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
阳性噬菌体展示肽也能与抗天然JEV抗原的鼠血清产生特异性反应。
The peptide displayed on positive phage could react specifically with the mouse antiserum against natural JEV Ag .
通过噬菌体肽库技术分析抗体所识别的抗原表位。
The mAb epitope was also analyzed using peptide phage display technology.
目的从噬菌体十二肽库中筛选出特异、灵敏的日本血吸虫病诊断抗原。
Objective To obtain the mimic epitope of specific and sensitive diagnostic antigen in schistosomiasis japonica from phage 12-mer peptide library.
目的从噬菌体十二肽库中筛选出特异、灵敏的日本血吸虫病诊断抗原。
Objective To obtain the mimic epitope of specific and sensitive diagnostic antigen in schistosomiasis japonica from phage 12-mer peptide library.
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