通过转化绿色荧光蛋白基因质粒,对阿特拉津降解基因工程菌进行标记。
The atrazine-degrading genetically engineered microorganism(GEM) was labeled by transforming a plasmid containing green fluorescent protein(GFP) gene.
另外,这些荧光蛋白基因还会随着癌细胞的复制而复制,因此这些癌细胞的后代也能发光。
In addition, the gene for the glowing protein is passed on by the cancer cells as they replicate, so that their progeny glow too.
以不同感染强度的携带绿色荧光蛋白基因重组腺病毒转染诱导后的脂肪细胞。
The adipose cells were transfected with Ad-GFP at the different multiplicity of infection (MOI).
他说,下一步是制造一个不在荧光蛋白基因位置断裂而是对疟原虫传播起关键作用的基因。
The next step, he says, is to make HEG break up not the fluorescent protein gene but one that is crucial for malaria transmission.
马丁chalfie证明的价值作为一个明亮的绿色荧光蛋白基因标记的各种生物现象。
Martin Chalfie demonstrated the value of GFP as a luminous genetic tag for various biological phenomena.
研究还显示,MAR序列能明显增强绿色荧光蛋白基因的表达能力(这一结果在另文讨论)。
The result also professes that the MAR sequence can enhance the expression of GFP gene significantly (data was shown in another paper).
目的通过对人退变髓核细胞的原代培养,了解其生物学特性,并将绿色荧光蛋白基因转染至细胞内。
Objective Human degenerative nucleus pulposus cells were cultured in monolayer, the biological action of the cells was observed.
通过使用DNA技术,研究人员现在可以连接到其他有趣的绿色荧光蛋白基因,但在其他无形,蛋白质。
By using DNA technology, researchers can now connect GFP to other interesting, but otherwise invisible, proteins.
本发明涉及生物技术中的基因工程领域,公开了一种含绿色荧光蛋白基因的植物转基因表达载体,以及其构建方法和应用。
The invention discloses a plant transgene expression carrier containing green fluorescent albumen gene, its method for constructing the same and use in genetic engineering field of biotechnology.
2000年,法国科学家将绿色荧光蛋白基因转接进兔子的基因组中;芝加哥的艺术家爱德华多·卡克(EduardoKac)宣称这是他的创意,不过后来科学家们反驳了他。
In 2000, French scientists spliced GFP into a white rabbit's genome; Chicago artist Eduardo Kac claimed it was his idea, though scientists later disputed that.
他们利用7个大肠杆菌菌株群,每个都加入了能表达出不同颜色荧光蛋白的基因。
The new scheme replaces the fuse with seven colonies of Escherichia coli bacteria, each given a gene for a different fluorescent protein.
这样极大提高了开发生物荧光的可能性,因为这意味着“绿色荧光蛋白”不再必须被注射到组织中,取而代之的是,其基因序列可以被加入到活体的基因中去。
This expanded the possibilities for exploiting bioluminescence dramatically, because it meant that GFP did not have to be injected into tissue.
绿色荧光蛋白(GFP)基因重组病毒标记技术是神经解剖研究的新方法。
The green fluorescence protein (GFP) gene recombinant virus labeling is a new method for the neuroanatomical studies.
目的构建携带报告基因增强型绿色荧光蛋白(EGFP)的反转录病毒载体,并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的构建以绿色荧光蛋白(GFP)为报告基因的酿酒酵母表达载体。
Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
方法:以绿色荧光蛋白(GFP)作为标记,观察hth_1基因在体外和脑内的表达。
Methods: By using GFP gene as a marker, to observe expression of human tyrosine hydroxylase type I (HTH1) gene in vitro or in brains.
近年来,分子生物学家门从中提取出了很多种荧光蛋白及它们的基因,并用基因工程建立了一系列具有不同发光特性的荧光蛋白。
Over recent years, molecular biologists have isolated a number of FPs and their genes, and used them to create genetically engineered variant FPs with particular light-emission characteristics.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中,脂质体法是效率高、安全性大的方法。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中,脂质体法是效率高、安全性大的方法。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
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