方法：免疫荧光组织化学双重标记技术。
METHODS: Immunofluorescence histochemical double-staining method was used.
方法免疫荧光组织化学双重染色，染色结果在激光共聚焦扫描显微镜下观察。
METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.
方法：应用荧光组织化学方法对青春期大鼠髁突软骨中的ER进行检测分析。
Methods: Luminescent histochemical method was used to detect the ER in the condylar cartilage in young growing SD rats.
方法：荧光金(FG)逆行追踪结合5 HT免疫荧光组织化学染色的双标技术。
METHODS: Fluoro Gold (FG) retrograde tracing combined with serotonin (5 HT) immunofluorescence histochemical staining method was used.
方法应用免疫荧光组织化学双标记染色技术，在激光共聚焦扫描显微镜下观察染色结果。
Methods Double label immunofluorescence histochemical staining and confocal laser_scanning microscope were used in the experiment.
结论视网膜血管消化铺片联合免疫荧光组织化学技术是可行的，为视网膜血管性病变的研究提供了重要的途径。
Conclusion retinal digest preparations combined with immunofluorescence. was feasible and provided an important method for the studies of retinal vascular diseases.
用免疫荧光组织化学法检测脑缺血再灌注后早期大鼠大脑梗死中心区、梗塞周边区和缺血对侧NG2和O4阳性细胞数量。
Then to determinate NG2 of O4 positive cells in the ischemic core, ischemic penumbra and contralateral area of rats at each time-point after reperfusion by immunofluorescence histochemistry.
方法SD大鼠单次全脑照射后用免疫荧光组织化学法分别检测早期大脑皮质和海马ca 1区NG2和O4阳性细胞数量。
Methods to determine, by immunofluorescence histochemical method, NG2 and O4-positive cells in the cortex and hippocampal CA1 area of SD rats early after single dose whole-brain irradiation.
方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。
Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.
结果免疫组织化学和双免疫荧光标记结果显示TIMP-1在正常肌肉的血管内皮细胞处表达；
Results The results of immunohistochemistry and double immunofluorescence showed that TIMP-1 was positive in vascular endothelial cells of normal muscles.
方法免疫组织化学和免疫荧光双标记技术。
Methods Immunohistochemical and double immunofluorescence techniques were used in the study.
电镜、相差显微镜、荧光显微镜检查及细胞组织化学染色均有助于诊断。
The electron microscope, phase contrast microscope, fluorescence microscope and cellular histochemical stain are helpful to diagnosis.
方法应用细胞培养、疫组织化学、荧光标记、光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
用荧光显微镜以及免疫组织化学方法观察HGF表达情况。
The expression of HGF protein was observed by fluorescence microscopy and immunohistochemistry.
方法应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
结果：免疫荧光和免疫组织化学法均表明成纤维细胞生长因子18在MDPC - 23细胞浆呈阳性表达。
RESULTS: Fibroblast growth factor 18 was expressed in cytoplasm of MDPC-23 cell by both immunohistochemical and immunofluorescent stainings.
免疫组织化学染色和免疫荧光染色提示角蛋白表达强阳性。
DOPA staining showed positive and immunohistochemical staining demonstrated that cells were stained by anti-human keratin.
两株抗体均可以采用细胞免疫荧光和流式细胞术检测ova66的表达，5f 4还可通过免疫组织化学检测肿瘤组织中ova66的表达。
These antibodies could be used for the detection of OVA66 expression in immunofluorescence and flow cytometry assay, whereas 5f4 could also be used in immunohistochemistry assay.
两株抗体均可以采用细胞免疫荧光和流式细胞术检测ova66的表达，5f 4还可通过免疫组织化学检测肿瘤组织中ova66的表达。
These antibodies could be used for the detection of OVA66 expression in immunofluorescence and flow cytometry assay, whereas 5f4 could also be used in immunohistochemistry assay.
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