目的:建立HPLC -荧光检测法同时测定大鼠血浆及脑组织中5种单胺类神经递质的方法。
Objective: To establish a method for determination of 5 monoamine neurotransmitters in blood and cerebral tissues of the rats by HPLC with fluorescence detection.
建立了灵敏度高、选择性好、可同时测定人血浆中右美沙芬活性代谢物去甲右美沙芬及愈创木酚甘油醚的HPLC-荧光检测法。
A new HPLC method with fluorescence detector was developed and validated for the simultaneous determination of dextrorphan and guaifenesin in human plasma.
STR体外酶活性分析采用间接荧光法检测色胺在反应体系的消耗。
In vitro, STR enzymatic assay was measured indirectly by fluorimetrically detecting depletion of tryptamine feeding on secologanin in the reaction mixture.
免疫荧光法检测神经元标志神经特异性烯醇化酶(nse)和神经丝蛋白(NF)的表达。
The expressions of neuronal markers NSE (neural specific enolase), NF (neurofilament), and glial cell marker GFAP (glial fibrillary acidic protein) were detected by fluorescent immunohistochemistry.
采用间接免疫荧光法(IFA)检测外源基因的表达。
The expression of exogenous genes were analyzed by indirect immunofluorescence assay (IFA).
目的通过与化学发光法比较,了解双色红外荧光技术在目标蛋白磷酸化检测中的优势。
Objective To assess the value of two-color infrared fluorescence imaging system in detecting protein phosphorylation in comparison with chemiluminescent detection.
采用间接免疫荧光法(IFA)检测外源基因的表达。
The exogenous gene expression was analyzed by indirect immunofluorescence assay (IFA).
对应用免疫荧光法检测鸡痘病毒感染鸡血清进行了研究。
The detection of chicken serum infected with henpox virus using the immunofluorescence method was studied.
实验结果表明荧光相图法可以检测蛋白质去折叠的中间态。
The experimental results show that the "phase diagram" method of fluorescence can be used to detect unfolding intermediates of proteins.
实验结果表明荧光相图法可以检测蛋白质去折叠的中间态。
The experimental results show that the 'phase diagram' method of fluorescence can be used to detect unfolding intermediates of proteins.
采用四氮甲基唑蓝比色法、溴乙锭荧光法检测肺细胞的生长情况和DNA交联的形成。
The cytotoxicity and DNA cross links of the cells were detected with MTT assay and ethidium bromide fluorescence assay.
建立了间接荧光免疫法检测纯化抗体的方法。
An indirect fluorescence immunoassay to detect antibody was developed.
相对荧光素酶活性法检测地塞米松作用后GR转录激活功能的变化。
The transcriptional activation function of GR was evaluated through the detection of relative luciferase activity after dexamethasone treatment.
内源荧光法以其灵敏度高、在线检测时间短、样品前处理简单等优势为微生物鉴别和表征提供了新方法。
The advantages of high sensitivity, rapid and simple operations make intrinsic fluorescence a powerful tool for reliable identification of bacteria.
间接免疫荧光法检测血清中抗核抗体(ANA)的荧光滴度。
The serum fluorescence titres of anti-nuclear antibodies (ANA) were tested by indirect immunofluorescence technique.
方法对疑似病例组织标本(皮肤、角膜、脑组织等)运用直接免疫荧光实验法检测狂犬病病毒抗原。
Methods rabies virus antigen was detected for the tissue samples (skin, cornea and brain) from the suspected rabies case by direct fluorescent antibody method (DFA).
结果表明:碘-碘化钾法、TTC法及改进TTC法、无机酸法和荧光染色法都不适合菊属植物花粉生活力的检测;
The results showed that the methods of iodine-iodide kalium, TTC or modified TTC, FDA, mineral acid were not able to detect the pollen viability of chrysanthemum.
用原子吸收光谱法、原子荧光光谱法和等离子发射光谱法,定性检测颗粒物中载带的重金属,并对其中9种重金属进行定量测定。
The heavy metals were detected with atomic absorption spectrum, atomic fluorescence spectrum and plasma emission spectrum, 9 heavy metals were measured quantitatively.
方法应用免疫荧光法检测60例肺癌患者、15例肺良性肿瘤患者和15例正常健康者外周静脉血T细胞亚群和NK细胞活性;
Methods The T subsets and NK cell from peripheral blood of 60 patients with lung cancer, 15 patients with lung benign tumor and 15 healthy people were detected by immunofluorescence.
收集鼠肺和鼠血,用间接免疫荧光法检测鼠肺中抗原和鼠血中抗体。
Rat blood and lungs were collected for indirect immunofluorescent detection of related antigens and antibodies.
方法用自动分析仪测定生理代谢参数,用免疫印迹和免疫荧光法检测glut1。
Methods Physiological parameters of myocardial metabolism were measured with automated analyzers, and GLUT1 distribution was assessed by immunoblot analysis and immunofluorescence.
方法采用连续采集法收集脑脊液样品,用HPLC荧光检测器测定各生物样品中MEP的浓度。
Methods CSF samples were collected by a serial sampling method. The concentration of MEP in the biological samples was measured by HPLC with fluorescence detector.
相对荧光素酶活性法检测GR转录激活功能的变化。
The transcriptional activation activity of GR was evaluated through method of relative activity of luciferase.
用高效液相色谱法,荧光检测器,同时检测鱼组织中恶喹酸、氟甲喹的残留量。
The high-performance liquid chromatography (HPLC) with fluorescence detector was used for the simultaneous determination of oxolinic acid and flumequine residues in fish tissues.
方法应用免疫荧光法对60例甲真菌病患者的外周血T细胞亚群进行检测。
Methods Peripheral T cell subsets in 60 patients with onychomycosis were detected by immunofluorescence.
方法采用间接免疫荧光法检测白血病患者T淋巴细胞亚群、NK细胞百分数,MTT法检测NK细胞杀伤活性。
Methods t lymphoid subgroups and the percentage of NK cells were detected by the use of indirect immunofluorescence method the function of NK cells was detected by MTT.
方法痰中病原菌定量培养、鉴定及荧光免疫法检测acb及其抗体类型。
METHODS Quantitative culture of pathogenic organisms in sputum samples and immunofluorescence method were used to detect ACBs and their types.
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ,综合分析各检测值对肝胃不和证积分的意义。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
了解小儿乙型肝炎病毒(HBVDNA)复制水平的变化及其临床意义。采用微粒子酶免分析法(MEIA)检测HBVM,荧光定量pcr法检h BV DNA。
To investigate the change of HBV DNA in children with hepatitis B. HBVM and HBV DNA were detected by microparticle enzyme immunoassay (MEIA) and PCR respectively.
了解小儿乙型肝炎病毒(HBVDNA)复制水平的变化及其临床意义。采用微粒子酶免分析法(MEIA)检测HBVM,荧光定量pcr法检h BV DNA。
To investigate the change of HBV DNA in children with hepatitis B. HBVM and HBV DNA were detected by microparticle enzyme immunoassay (MEIA) and PCR respectively.
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