这说明荧光定量RT-PCR方法可以对临床上H5N1虎源流感病毒检测提供参考。
The result indicated that fluorogenetic quantitation RT-PCR may be used as a good reference in detection of H5N1 TIV.
目的探讨荧光定量聚合酶链反应（FQ-PCR）技术诊断结核病临床应用价值。
Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis.
目的探讨荧光定量聚合酶链反应(FQ - PCR)在巨细胞病毒性肝炎中的应用价值。
Objective To investigate the fluorescence quantitative polymerase chain reaction (FQ-PCR) in the giant cell viral hepatitis in value.
方法应用荧光定量聚合酶链反应(FQ - PCR)对尖锐湿疣高危人群分泌物标本进行HPV - DNA分型检测。
Methods the secretion specimen coming from the high risk patients with CA were examined with fluorescent quantitative polymerase chain reaction (FQ-PCR) for genotype HPV-DNA.
结果：荧光定量方法可以快速完成MTHFR C677T基因多态性的检测，检测结果得到传统PCR-RFLP方法的证实。
Results : Detection of MTHFR gene C677T polymorphism was quickly finished by realtime fluorescence quantitative RT - PCR, and the results were proved to be effective by PCR - RFLP.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
目的探讨荧光定量聚合酶链反应(FQ PCR)与常规聚合酶链反应检测乙型肝炎病毒(HBV)敏感性的差异。
Objective To explore the sensitivity difference between fluorescent quantity PCR (FQ-PCR) and routine PCR for HBV DNA detection.
使用动力学数学模型做定量pcr分析，其结果的误差仅与使用的荧光强度数值的精确度相关。
Using this model to do quantitative PCR analysis, result error is only related to the accuracy of fluorescence intensity or the instrument used.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ，综合分析各检测值对肝胃不和证积分的意义。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
目的：用荧光定量聚合酶链反应(FQ - PCR)测定丙型肝炎病毒在临床的应用。
Objective To investigate the clinical application of the Fluorescence quantitative PCR (FQ-PCR) in the detection of HCV infection.
方法将接受拉米夫定治疗的188例患者根据治疗时间进行分组，采用实时荧光PCR方法定量检测各组患者血清HBVDNA水平和酪氨酸-甲硫氨酸-天冬氨酸-天冬氨酸（YMDD）变异。
Methods Serum samples from 188 chronic hepatitis B patients with lamivudine therapy were collected and quantitatively tested with real-time PCR for HBV DNA and YMDD mutations.
变形链球菌；荧光定量PCR；唾液；
目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。
Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.
分别采用流式细胞计数仪和荧光定量PCR法检测慢性乙型肝炎患者外周血淋巴细胞凋亡率与血清HBVDNA。
The apoptosis of peripheral lymphocytes extracted from chronic hepatitis B patients were analyzed by flow cytometer. Serum HBVDNA was detected by quantitatively PCR.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
目的探讨荧光定量pcr (FQPCR)检测细胞角蛋白19 (ck19)在乳腺癌诊断中的应用。
Objective to evaluate the clinical application of fluorescent quantification polymerase chain reaction (FQ PCR) to detect cytokeratin 19 (CK19) in the diagnosis of breast cancer.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
前言： 目的：评估荧光定量PCR在检测胃粘膜上幽门螺杆菌的DNA中的应用价值。
Objective:To evaluate the clinical application value of FQ-PCR(fluorogenic quantitative polymerase chain reaction) for detecting HP DNA in human gastric mucosa.
目的：探讨脂血、高胆红素和溶血标本对乙肝病毒DNA（HBVDNA）荧光定量测定结果的影响。
Objective: To investigate the effect of the high lipid, high bilirubin and hemolytic blood specimens on real time fluorimetry PCR of HBVDNA.
将人禽流感死亡病例的尸解标本，包括心、肝、脾、肺、肾、脑等组织标本研磨处理后，进行荧光定量pcr检测，并根据标准曲线推算H5N1禽流感病毒的病毒载量。
The autopsy samples, including heart, liver, spleen, lung, kidney and brain, were ground and detected by Real-time PCR. According to the standard curve, virus load of H5N1 AIV was calculated.
文章对实时荧光定量pcr的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.
并通过荧光定量PCR研究其在外施五种激素的条件下的胶乳中的表达谱。
The real time quantitative PCR is applied to research the expression trend of these genes under five different hormones.
目的运用终点法荧光定量pcr检测HBV -DNA，探讨其临床应用价值。
Objective To investigate the clinical value of endpoint detection of HBV-DNA by means of fluorescent quantitive PCR.
建立一种利用荧光定量pcr扩增反应进行单核苷酸多态性(snp)快速测定的方法。
A new effective single nucleotide polymorphism (SNP) typing method was developed by using real-time polymerase chain reaction (PCR).
目的探讨实时荧光定量pcr检测巨细胞病毒在肾移植术后肺部感染诊断中的应用价值。
Objective to examine the value of real time PCR in the diagnosis of cytomegalovirus pneumonia in renal Allograft Recipient.
构建汉坦病毒S片段标准品，用于实时荧光定量pcr检测汉坦病毒。
To explore the method of preparation for Hantavirus s segment standard plasmids of real-time PCR.
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