这说明荧光定量RT-PCR方法可以对临床上H5N1虎源流感病毒检测提供参考。
The result indicated that fluorogenetic quantitation RT-PCR may be used as a good reference in detection of H5N1 TIV.
目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
目的：利用时间分辨荧光免疫分析(TRFIA)技术，建立人血清ck - MB的快速定量检测方法。
AIM: To make a fast quantitative detection of Creatine Kinase-MB Isoenzyme (CK-MB) based on time-resolved fluoroimmunoassay (TRFIA).
方法应用荧光定量聚合酶链反应(FQ - PCR)对尖锐湿疣高危人群分泌物标本进行HPV - DNA分型检测。
Methods the secretion specimen coming from the high risk patients with CA were examined with fluorescent quantitative polymerase chain reaction (FQ-PCR) for genotype HPV-DNA.
目的探讨荧光定量pcr (FQPCR)检测细胞角蛋白19 (ck19)在乳腺癌诊断中的应用。
Objective to evaluate the clinical application of fluorescent quantification polymerase chain reaction (FQ PCR) to detect cytokeratin 19 (CK19) in the diagnosis of breast cancer.
目的探讨用荧光定量PCR检测不明原因流产组织及血清中巨细胞病毒（CMV）和弓形体（TOX）感染。
Objective: Detect the infection of CMV and TOX in tissue , serum of unexplained abortion by FQ-PCR.
将人禽流感死亡病例的尸解标本，包括心、肝、脾、肺、肾、脑等组织标本研磨处理后，进行荧光定量pcr检测，并根据标准曲线推算H5N1禽流感病毒的病毒载量。
The autopsy samples, including heart, liver, spleen, lung, kidney and brain, were ground and detected by Real-time PCR. According to the standard curve, virus load of H5N1 AIV was calculated.
方法运用荧光定量pcr (FQ - pcr)和el IS A两种方法同时检测了310份肝炎患者血清，并对结果进行了对比分析。
Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay (FQ PCR), and also by ELISA as contrast.
定量荧光检测技术克服了常规荧光录井中的种种不足，是一种先进的油层监测技术。
Quantitative fluorescence detecting technology is an advanced reservoir detecting technology which overcomes various defects in normal fluorescence logging.
结果：荧光定量方法可以快速完成MTHFR C677T基因多态性的检测，检测结果得到传统PCR-RFLP方法的证实。
Results : Detection of MTHFR gene C677T polymorphism was quickly finished by realtime fluorescence quantitative RT - PCR, and the results were proved to be effective by PCR - RFLP.
目的建立灵敏、稳定、特异的实时荧光PCR方法，用于戊型肝炎病毒的定量检测。
To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.
结论荧光定量PCR法在检测沙眼体较金标法更敏感、快速，是早期诊断生殖道沙眼衣原体感染的一种极有价值的方法。
Conclusion: It is suggested that PCR in detecting chlamydozoa trachomatis appears to be more sensitive and more rapid than gold-labelled antigen detection method.
前言： 目的：评估荧光定量PCR在检测胃粘膜上幽门螺杆菌的DNA中的应用价值。
Objective:To evaluate the clinical application value of FQ-PCR(fluorogenic quantitative polymerase chain reaction) for detecting HP DNA in human gastric mucosa.
方法将接受拉米夫定治疗的188例患者根据治疗时间进行分组，采用实时荧光PCR方法定量检测各组患者血清HBVDNA水平和酪氨酸-甲硫氨酸-天冬氨酸-天冬氨酸（YMDD）变异。
Methods Serum samples from 188 chronic hepatitis B patients with lamivudine therapy were collected and quantitatively tested with real-time PCR for HBV DNA and YMDD mutations.
方法痰中病原菌定量培养、鉴定及荧光免疫法检测acb及其抗体类型。
METHODS Quantitative culture of pathogenic organisms in sputum samples and immunofluorescence method were used to detect ACBs and their types.
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ，综合分析各检测值对肝胃不和证积分的意义。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
系统应用在化学荧光免疫定量检测仪中，实际运行结果表明：该系统稳定性好，精确度高，达到了预期的性能指标要求。
The system is working well in the chemiluminescent fluorescence immunoassay detector with high stability and precision, and can reach the performance that expected.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
分别采用流式细胞计数仪和荧光定量PCR法检测慢性乙型肝炎患者外周血淋巴细胞凋亡率与血清HBVDNA。
The apoptosis of peripheral lymphocytes extracted from chronic hepatitis B patients were analyzed by flow cytometer. Serum HBVDNA was detected by quantitatively PCR.
为了探讨氯化铈对肿瘤细胞凋亡的影响，本实验以K562细胞为研究对象，采用荧光显微镜定性检测细胞凋亡、流式细胞术定量测定细胞凋亡率。
To investigate the apoptotic effect in K562 cells exposed to cerium chloride, apoptosis was examined using flow cytometry and apoptosis morphology was detected with fluorescence microscopy.
用原子吸收光谱法、原子荧光光谱法和等离子发射光谱法，定性检测颗粒物中载带的重金属，并对其中9种重金属进行定量测定。
The heavy metals were detected with atomic absorption spectrum, atomic fluorescence spectrum and plasma emission spectrum, 9 heavy metals were measured quantitatively.
方法用酶联免疫吸附试验对982例乙肝患者血清标志物和乙肝病毒前S2抗原进行检测；并用荧光定量PCR法对其进行HBV-DNA检测。
Methods Hepatitis B serum markers and Pre-S2 antigens were tested by enzyme-linked immunosorbent assay and HBV DNA was detected by fluorescent quantitative-PCR in 982 hepatitis B patients.
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
目的探讨荧光定量聚合酶链反应(FQ PCR)与常规聚合酶链反应检测乙型肝炎病毒(HBV)敏感性的差异。
Objective To explore the sensitivity difference between fluorescent quantity PCR (FQ-PCR) and routine PCR for HBV DNA detection.
目的运用终点法荧光定量pcr检测HBV -DNA，探讨其临床应用价值。
Objective To investigate the clinical value of endpoint detection of HBV-DNA by means of fluorescent quantitive PCR.
目的：在干眼症治疗过程中应用丫啶橙荧光染色法进行结膜印迹细胞学定量检测，了解病变结膜细胞恢复状况。
Aim to evaluate the functional condition of corneal cells and to detect the degree of keratoconjunctivitis sicca using conjunctival impression cell test with acridine orange fluorescent staining.
目的探讨实时荧光定量pcr检测巨细胞病毒在肾移植术后肺部感染诊断中的应用价值。
Objective to examine the value of real time PCR in the diagnosis of cytomegalovirus pneumonia in renal Allograft Recipient.
构建汉坦病毒S片段标准品，用于实时荧光定量pcr检测汉坦病毒。
To explore the method of preparation for Hantavirus s segment standard plasmids of real-time PCR.
了解小儿乙型肝炎病毒(HBVDNA)复制水平的变化及其临床意义。采用微粒子酶免分析法(MEIA)检测HBVM，荧光定量pcr法检h BV DNA。
To investigate the change of HBV DNA in children with hepatitis B. HBVM and HBV DNA were detected by microparticle enzyme immunoassay (MEIA) and PCR respectively.
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