建立了间接荧光免疫法检测纯化抗体的方法。
An indirect fluorescence immunoassay to detect antibody was developed.
方法痰中病原菌定量培养、鉴定及荧光免疫法检测acb及其抗体类型。
METHODS Quantitative culture of pathogenic organisms in sputum samples and immunofluorescence method were used to detect ACBs and their types.
采用间接免疫荧光法(IFA)检测外源基因的表达。
The expression of exogenous genes were analyzed by indirect immunofluorescence assay (IFA).
免疫荧光法检测神经元标志神经特异性烯醇化酶(nse)和神经丝蛋白(NF)的表达。
The expressions of neuronal markers NSE (neural specific enolase), NF (neurofilament), and glial cell marker GFAP (glial fibrillary acidic protein) were detected by fluorescent immunohistochemistry.
抗甲状腺球蛋白免疫荧光法阳性的病例。
Here is an example of immunofluorescence positivity for anti-thyroglobulin antibody.
采用间接免疫荧光法(IFA)检测外源基因的表达。
The exogenous gene expression was analyzed by indirect immunofluorescence assay (IFA).
目的对荧光偏振免疫法(FPIA)测定血浆同型半胱氨酸(HCY)的方法学进行初步评价。
Objective To evaluate the detection of plasma homocysteine (HCY) with fluorescence polarization immunoassay (FPIA) technology.
其敏感性比间接免疫荧光法（IFA）高数十倍；
用MTT方法对脾脏的NK杀伤细胞率和淋巴细胞的转化率进行测定，采用免疫荧光法对CD4+、CD8+细胞进行测定。
The killing activity of NK cells and the proliferation rate of spleen cells were tested by MTT assay, and T cell subgroupCD4+ , CD8+ were tested by the indirect immune fluorescent assay.
对应用免疫荧光法检测鸡痘病毒感染鸡血清进行了研究。
The detection of chicken serum infected with henpox virus using the immunofluorescence method was studied.
结果：经荧光显微镜、流式细胞仪及免疫印迹法检测，证实AIRE基因得到了稳定表达。
Results: Stable expression of AIRE was confirmed by fluorescence microscope, FACS and western blotting analysis.
目的探讨荧光酶免疫法（FEIA）在新生儿甲状腺功能低下症筛查中的实用性。
Objective To discuss the availability of fluorescent enzyme immunoassay (FEIA) in neonatal diseases screening.
方法对疑似病例组织标本(皮肤、角膜、脑组织等)运用直接免疫荧光实验法检测狂犬病病毒抗原。
Methods rabies virus antigen was detected for the tissue samples (skin, cornea and brain) from the suspected rabies case by direct fluorescent antibody method (DFA).
目的建立亚马逊利什曼原虫无鞭毛体体外培养方法，并用间接免疫荧光法分析其抗原特异性。
Objective To establish a method for culture of Leishmania amazonensis amastigotes in vitro and identify the antigenic specificity of amastigotes with indirect immunofluorescent assay.
然后通过免疫荧光法，观察对登革病毒复制的阻断作用。
The blocking of dengue virus replication was observed by immunofluorescence.
方法：使用荧光偏振免疫法监测地高辛血浓度，并统计分析年龄、疾病、合并用药等因素对地高辛血浓度的影响。
Method: the digoxin blood concentration was monitored by fluorescence polarizing immunization, the influence factors, such as age, disease, and combined with drug, were analysed.
方法：采用间接免疫荧光法、小鼠保护试验以及SDS -聚丙烯胺电泳法，分别测定分离株的血清类型、毒力和蛋白图谱。
Method: Use the method of indirect immunofluorescence, mice cross protection test and SDS -page to determine the serum type, the toxicity and the protein atlas of the isolated strains.
方法应用免疫荧光法检测60例肺癌患者、15例肺良性肿瘤患者和15例正常健康者外周静脉血T细胞亚群和NK细胞活性；
Methods The T subsets and NK cell from peripheral blood of 60 patients with lung cancer, 15 patients with lung benign tumor and 15 healthy people were detected by immunofluorescence.
方法应用MTT、CFU - L及间接免疫荧光法对18例急性白血病进行观察。
Methods MTT, CFU-L and indirect immune fluorescence were used in 18 patients with acute leukemias.
间接免疫荧光法检测血清中抗核抗体(ANA)的荧光滴度。
The serum fluorescence titres of anti-nuclear antibodies (ANA) were tested by indirect immunofluorescence technique.
目的：建立快速测定茶碱血清浓度的高效液相色谱(hplc)法，研究应用此法和常规的荧光偏振免疫分析(FPIA)法测定结果的相关性。
Aim: To establish a rapid HPLC method determining serum theophylline concentration and research the relativity of results determined by HPLC and by routine FPIA.
方法：用直接免疫荧光法查检鼠肺中的病毒抗原。
Methods: Using direct immunofluorescence assay (DIFA) to detect EHF virus from the lungs of rodents.
免疫荧光法鉴定脂肪基质干细胞表达CD13和CD44。
The adipose stem cells expressed CD13 and CD44 through immunofluorescence.
微粒子酶联免疫荧光法（MEIA）检测大肠癌患者术前血浆CEA、CA19-9水平。
The plasma levels of CEA and CA19-9 were determined by microparticle enzyme-linked immunoassay (MEIA) before operation.
用胰腺冰冻切片间接免疫荧光法成功地检测出血清中胰岛细胞抗体(ICA)。
Islet cell antibodies (ICA) were successfully detected with indirect immunofluorescence assay on frozen sections of human pancreas.
结果免疫荧光法显示注射侧黑质致密部TH阳性细胞数和网状部TH阳性纤维面积与正常侧的百分比平均值分别为21.83%，23.19%。
Results the average percent of th immunoreactive cells and the area of th immunoreactive fibers in the SN of the injected side were 21.83% and 23.19% compared with that of the normal side.
方法采用间接免疫荧光法检测白血病患者T淋巴细胞亚群、NK细胞百分数，MTT法检测NK细胞杀伤活性。
Methods t lymphoid subgroups and the percentage of NK cells were detected by the use of indirect immunofluorescence method the function of NK cells was detected by MTT.
继用抗S-100蛋白单抗通过间接免疫荧光法及ABC法进行细胞鉴定。
The cells were identified with anti S100 protein antibody by indirect immunofluoresent methods and ABC methods.
方法用自动分析仪测定生理代谢参数，用免疫印迹和免疫荧光法检测glut1。
Methods Physiological parameters of myocardial metabolism were measured with automated analyzers, and GLUT1 distribution was assessed by immunoblot analysis and immunofluorescence.
方法：利用荧光偏振免疫分析法(FPIA)对135例癫痫病人进行PB血药浓度监测，并对测定结果进行分析。
Methods: 135 epilepsy patients were monitored the PB blood concentrations by FPIA method and the result were analyzed.
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