用时间分辨荧光免疫分析技术（TRFIA）对乙肝五项定量分析；
Time-resolved fluoroimmune assay(TRFIA) was used to measure the quantitation of HBV markers in serum.
目的对本所研制的AFP时间分辨荧光免疫分析试剂盒进行方法学评价。
Objective To evaluate the methodology for AFP with time-resolved fluoroimmunoassay established by jiangsu institute of nuclear medicine.
目的：利用时间分辨荧光免疫分析(TRFIA)技术，建立人血清ck - MB的快速定量检测方法。
AIM: To make a fast quantitative detection of Creatine Kinase-MB Isoenzyme (CK-MB) based on time-resolved fluoroimmunoassay (TRFIA).
时间分辨荧光免疫分析技术作为新一代非放射性免疫分析技术，具有生物技术研究和临床应用的广阔前景。
Time-resolved fluorescence immunoassay technology is a new generational non -radioactivity immunoassay. It has wide application and bright foreground in biotechnology and clinical detection.
现代荧光免疫分析技术主要包括荧光偏振免疫分析(FPIA)和时间分辩荧光免疫分析(TRFIA)两种技术。
Modern fluorescence immunoassay, which includes fluorescence polarization immunoassay (FPIA) and time-resolved fluorescence immunoassay (TRFIA), also plays an important role in these subjects.
免疫荧光共定位分析用于检查突变蛋白的合成和分泌。
Immunofluorescence co-localization analysis was used to examine the synthesis and secretion of the mutant protein.
方法对8227例产前孕妇进行血清afp的测定，方法用时间分辨免疫荧光分析法。
Methods 8227 serum AFP specimens were detected from prenatal screening gravidas by TRFIA.
方法：使用荧光偏振免疫法监测地高辛血浓度，并统计分析年龄、疾病、合并用药等因素对地高辛血浓度的影响。
Method: the digoxin blood concentration was monitored by fluorescence polarizing immunization, the influence factors, such as age, disease, and combined with drug, were analysed.
方法骨髓有核细胞免疫荧光标记后用流式细胞术分析。
Methods Bone marrow nuclear cells (BMNCs) were labeled by immunofluorescence and analyzed by flow cytometry.
方法采用三色免疫荧光直接标记法及多参数流式细胞术分析13例多发性骨髓瘤的免疫表型。
Methods Three-colour direct immunofluorescence staining and multiparameter flow cytometry were used for analysis of 13 samples of immunophenotyping of multiple myeloma patients.
方法：采用间接免疫荧光流式细胞术，分析K562细胞表面TRAIL分子的表达。
METHODS: The expression of TRAIL on K562 cells was detected by indirect immunofluorescence staining and flow cytometry.
二抗的最佳稀释度根据实际应用来确定。最终稀释度为1：500时，对于大多数的免疫荧光分析都能得出可取的结果。
The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most immunofluorescent applications.
并采用免疫荧光抗体染色技术对5例dmd,2例BMD肌细胞膜上抗肌营养不良蛋白的表达观察分析，以2例正常人的肌组织作为对照。
The patients from 5 DMD and 2 BMD were detected by immunofluorescence technique for analyzing dystrophin located in muscle cell membrane, compared with 2 normal males.
目的建立亚马逊利什曼原虫无鞭毛体体外培养方法，并用间接免疫荧光法分析其抗原特异性。
Objective To establish a method for culture of Leishmania amazonensis amastigotes in vitro and identify the antigenic specificity of amastigotes with indirect immunofluorescent assay.
荧光共振能量转移(FRET)技术作为一种高效的光学“分子尺”，在生物大分子相互作用、免疫分析、核酸检测等方面有广泛的应用。
Fluorescence resonance energy transfer (FRET) has broad applications in the study of the interactions of biological macromolecules, immunoassay, nucleic acid detection and so on.
分析彩色免疫荧光图像可以获得细胞免疫信息，用于辅助研究和医疗诊断。
Analyzing color immunofluorescence images can get immunological information of cells and provide an auxiliary method of research and disease diagnosis.
方法：采用间接免疫荧光标记，流式细胞术分析。
Methods Indirect immune fluorescent labeling and flow cytometry were employed.
方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。
Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.
方法用自动分析仪测定生理代谢参数，用免疫印迹和免疫荧光法检测glut1。
Methods Physiological parameters of myocardial metabolism were measured with automated analyzers, and GLUT1 distribution was assessed by immunoblot analysis and immunofluorescence.
目的：建立快速测定茶碱血清浓度的高效液相色谱(hplc)法，研究应用此法和常规的荧光偏振免疫分析(FPIA)法测定结果的相关性。
Aim: To establish a rapid HPLC method determining serum theophylline concentration and research the relativity of results determined by HPLC and by routine FPIA.
方法：采用受体放射配基结合分析、受体-DNA结合分析及免疫荧光技术。
Methods: receptor radioligand binding assay, receptor DNA binding assay and immunofluorescence methods were used.
方法：采用荧光偏振免疫法测定276例癫痫患者的丙戊酸血药浓度并分析结果。
METHODS: the serum concentrations of valproate in 276 patients were determined by fluorescence polarization immunoassay and the results were analyzed.
抗体的最佳稀释度取决于每一个应用。然而，1：1000的终稀释度在免疫荧光分析中可以得到好的结果。
The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescentapplications.
免疫荧光染色后细胞纯度分析显示，A2B5染色阳性细胞占DAPI染核细胞的百分比为98.14%。
Purity analysis after immunofluorescence staining revealed that the ratio of number of A2B5-positive cells to that of DAPI-positive cells was 98.14%.
方法：利用荧光偏振免疫分析法(FPIA)对135例癫痫病人进行PB血药浓度监测，并对测定结果进行分析。
Methods: 135 epilepsy patients were monitored the PB blood concentrations by FPIA method and the result were analyzed.
方法采用间接免疫荧光法分析102例初治all患者的免疫表型。
Method Immunophenotypes were examined using indirect immunofluorescence method in 102 denovo ALL.
方法：采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
为了评价免疫分型在急性白血病（AL）分型中的科学意义，应用形态学分型、直接免疫荧光标记流式细胞仪检测免疫分型对68例AL进行了分析。
To evaluate the significance of immunologic classification for typing of acute leukemia (AL). 68 cases of AL were classified by morphologic and immunologic typings.
为了评价免疫分型在急性白血病（AL）分型中的科学意义，应用形态学分型、直接免疫荧光标记流式细胞仪检测免疫分型对68例AL进行了分析。
To evaluate the significance of immunologic classification for typing of acute leukemia (AL). 68 cases of AL were classified by morphologic and immunologic typings.
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