方法:采用常规膜片钳全细胞记录技术。
Method: With conventional Patch Clamp whole-cell recording technique.
结果:(1)建立了膜片钳全细胞记录技术平台,获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
方法:采用全细胞膜片钳技术,记录钠通道电流。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法传代得到单个HERG-HEK细胞,应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线(激活、失活、复活和去活化)。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法:运用全细胞膜片钳技术记录持续性钠电流,并观察其在缺氧-复氧模型下的变化。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
方法用急性酶解法获得单个大鼠心室肌细胞,标准的全细胞膜片钳技术记录钠通道电流。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法应用全细胞膜片钳技术记录乌头碱对大鼠心肌细胞动作电位时程的作用,分别给予奎尼丁、维拉·帕米处理比较二者对动作电位时程的影响。
Methods Effects of quinidine and verapamil on the alteration of action potential duration induced by aconitine were studied by use of the whole cell patch clamp techniques in rat ventricular myocytes.
采用全细胞膜片钳技术,记录动作电位和上述各离子流。
We used current-clamp technique to record action potentials, and voltage-clamp technique to record ionic currents.
方法应用膜片钳技术全细胞构型从原代培养的螺旋神经元获得电流钳记录。
Methods Current-clamp recordings with the use of the whole cell configuration of the patch-clamp technique were made from the primary cultured spiral ganglion neurons.
方法用急性酶解法获得单个大鼠心室肌细胞,标准的全细胞膜片钳技术记录钠通道电流。
MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.
用全细胞模式膜片钳技术记录分析血管平滑肌细胞L型电压依赖性钙通道的特性。
The electric physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique (PCT).
方法:采用全细胞膜片钳技术记录蟾蜍灵激活cne - 2 Z细胞膜电流并分析其电流特征。
METHODS: the technique of whole-cell patch clamp was used to record the chloride currents and to analyze the characteristics of the currents in CNE-2Z cells.
方法:采用全细胞膜片钳技术记录蟾蜍灵激活cne - 2 Z细胞膜电流并分析其电流特征。
METHODS: the technique of whole-cell patch clamp was used to record the chloride currents and to analyze the characteristics of the currents in CNE-2Z cells.
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