• 方法采用常规膜片全细胞记录技术

    Method: With conventional Patch Clamp whole-cell recording technique.

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  • 结果:(1)建立膜片全细胞记录技术平台,获得了稳定性正常心室细胞细胞离子通道电流图。

    Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.

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  • 方法采用全细胞膜片技术记录通道电流

    Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.

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  • 方法传代得到单个HERG-HEK细胞,应用全细胞膜片技术记录HERG-HEK细胞上的HERG钾电流动力学曲线(激活、失活、复活和去活化)。

    Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.

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  • 方法运用细胞膜片技术记录持续性钠电流观察缺氧-复氧模型变化

    Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.

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  • 方法急性酶解法获得单个大鼠心室肌细胞,标准全细胞膜片技术记录通道电流

    The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.

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  • 方法应用细胞膜片技术记录头碱对大鼠心肌细胞动作电位时程作用,分别给予奎尼丁维拉·帕米处理比较二者动作电位时程的影响

    Methods Effects of quinidine and verapamil on the alteration of action potential duration induced by aconitine were studied by use of the whole cell patch clamp techniques in rat ventricular myocytes.

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  • 采用全细胞膜片钳技术记录动作电位上述各离子流

    We used current-clamp technique to record action potentials, and voltage-clamp technique to record ionic currents.

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  • 方法应用膜片技术细胞构型代培养螺旋神经元获得电流记录

    Methods Current-clamp recordings with the use of the whole cell configuration of the patch-clamp technique were made from the primary cultured spiral ganglion neurons.

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  • 方法急性酶解法获得单个大鼠心室肌细胞,标准全细胞膜片技术记录钠通道电流

    MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.

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  • 细胞模式膜片技术记录分析血管平滑肌细胞L电压依赖性通道特性

    The electric physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique (PCT).

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  • 方法采用细胞膜片技术记录蟾蜍灵激活cne - 2 Z细胞电流分析电流特征

    METHODS: the technique of whole-cell patch clamp was used to record the chloride currents and to analyze the characteristics of the currents in CNE-2Z cells.

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  • 方法采用细胞膜片技术记录蟾蜍灵激活cne - 2 Z细胞电流分析电流特征

    METHODS: the technique of whole-cell patch clamp was used to record the chloride currents and to analyze the characteristics of the currents in CNE-2Z cells.

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