人IL-2基因腺病毒载体的构建和包装。
文章综述了近年来牛腺病毒载体构建的一些研究进展。
The paper reviewed progress on the construction of bovine adenovirus vectors.
目的构建含有人TRADD基因片段的重组腺病毒载体。
Objective to construct recombinant adenovirus vector carrying human TRADD gene.
目的：比较鞘内注射第一代与第三代腺病毒载体后引起免疫反应的差异。
Objective:To examine the different immune responses between the first-generation and the third-generation Ad vectors by intrathecal injection.
目的：构建携带内皮抑素基因的增殖型腺病毒载体，寻找结肠癌的有效治疗方法。
Objective: To construct a replication-competent adenovirus vector carrying human endostatin gene, looking for an effective method for the therapy of colon cancer.
结论：构建的心肌特异性表达腺病毒载体可使外源基因在心肌细胞内特异性表达。
Conclusion: the established viral vector AdmlcGFP allows heart specific expression of a foreign gene.
通过腺病毒载体介导使多药耐药基因转染入脐血有核细胞，提高其对化疗药物的耐受性。
MDR1 gene was transfered to cord blood nucleate cells (CBNC) mediated by adenovirus vector, for enhancing its resistance to chemotherapeutic drugs.
目的研究携带血管抑素K1-5基因的腺病毒载体对其对血管内皮细胞增殖的抑制作用。
Objective To investigate the suppression effects of a adenovirus vector containing angiostatin K1-5 gene to the proliferation and migration of vascular endothelial cells.
目的：建立一种在甲胎蛋白(afp)阳性的肝癌细胞中靶向表达目的基因的重组腺病毒载体。
AIM: to construct a recombinant adenovirus vector carrying AFP promoter to specifically express a targeting gene in hepatocellular carcinoma HepG2 cells.
默克疫苗的失败至多代表非复制型腺病毒载体疫苗的失败，决不代表其他艾滋病疫苗就没有希望。
The failure of the Merck vaccine up on behalf of the non-replicating adenovirus vector vaccine failure, not on behalf of other AIDS vaccines will be no hope.
摘要腺病毒载体具有高效转染和低细胞毒性的特点，已成为基因治疗研究中应用最广泛的载体之一。
However, the clinical application of the third-generation adenovirus vectors is hampered by the existance of helper contamination and difficulties in their large-scale production.
目的构建携带反义热休克蛋白70 (HSP70)的重组腺病毒载体以用于喉癌的基因治疗研究。
Objective to construct a recombinant adenovirus vector carrying antisense heat shock protein 70 (HSP70) cDNA and observe its effect on inhibiting the growth of laryngeal carcinoma Hep-2 cells.
摘要腺病毒载体具有高效转染和低细胞毒性的特点，已成为基因治疗研究中应用最广泛的载体之一。
Adenovirus vectors have the characteristics of high transfection efficiency and low cytotoxicity, and they have become most widely used vectors for gene therapy.
结论成功地构建了携带CCR5反义rna重组腺病毒载体，为研究其抗hiv 1的作用打下基础。
Conclusion The recombinant adenovirus carrying antisense RNA to CCR5 has laid down a good foundation for studying its inhibiting effect on HIV-1 infection.
腺病毒载体是继逆转录病毒载体后在基因治疗、基因免疫等方面应用开发得较早且较成熟的一种基因载体。
Adenovirus vector is an earlier and maturer genetic carrier in the respect of gene therapy and genetic immunization after retroviral vector.
目前应用的病毒载体主要有逆转录病毒载体、腺病毒载体、腺病毒相关病毒、慢病毒载体、单纯疱疹病毒载体等。
At present, the mainly applied virus vectors are retroviral vector, adenovirus vector adenovirus-associated virus slow virus vector, herpes simplex virus vector and so on.
本发明还涉及人源化改造的基因突变技 术和去除GFP的腺病毒转移载体及去除GFP的同源重组腺病毒载体。
The invention also relates to a human reforming gene mutation technique, adenovirus transition carrier removing GFP and homologous recombinant adenovirus carrier.
在腺病毒载体的构建过程中，早期和晚期启动子的选择、构建以及去除末端蛋白(TP)、克隆末端序列是至关重要的。
The selection and cloning of early and late promoters and removal of adenovirus terminal protein (TP) are very important in construction of adenovirus vector.
结论利用位置特性重组技术，成功的构建了人反义B7 - H1的腺病毒载体，为后续对B7 - H1的相关研究创造了条件。
Conclusion the recombinant adenovirus vector was constructed successfully by site-specific recombination technique, which provides a foundation for further research about B7-H1.
在此，我们研究了用白介素(IL) - 13进行腺病毒载体基因治疗的效果，白介素(IL) - 13是一种在急性肾移植损伤中有强大免疫调节特性的细胞因子。
Here we investigate the effects of adenovirus-mediated gene therapy with interleukin (IL) -13, which is a cytokine with strong immunomodulatory properties, on acute renal allograft injury.
几年来，专家致力于将腺病毒作为一个载体将治疗基因带入细胞，大多是为了治疗癌症以及心血管疾病。
For several years, scientists have tried to use adenoviruses as a vector to carry therapeutic genes into cells, mostly for treating cancer and cardiovascular disease.
Chiu补充说，伶猴腺病毒(TMAdV)在人类身上罕见，这让它可能成为一个潜在的强大工具，在基因治疗中充当病毒载体。
TMAdV's rarity in humans could make it a potentially powerful tool as a viral vehicle for delivering gene therapy, Chiu adds.
人们希望，改构后的腺病毒在基因治疗方面能起到重要的作用，例如，作为载体基因携带治疗性基因到达细胞内。
The hope is that modified adenovirus could play a role in gene therapy, used as a vector (carrier) for delivering therapeutic genes to the interior of cells.
目的：构建含人IL -21基因的重组腺病毒表达载体，为IL - 21基因治疗肿瘤研究奠定基础。
Objective: to be as the foundation of the tumor therapy with targeting IL-21 gene by constructing of recombinant interleukin 21 gene adenovirus expression vector.
目的尝试利用腺病毒介导慢病毒载体的细胞感染以提高转染效率。
Purpose To test the hypothesis that adenovirus may improve gene transfer efficiency of lentiviral vector by mediating viral entry.
目的：克隆人和小鼠的活化t细胞表达与分泌调节基因(RANTES基因)并分别构建腺病毒表达载体。
Objective: to clone the human and murine origin RANTES genes and construct the adenoviral expression vectors.
腺病毒作为新兴的载体因其转染效率和表达效率高而日益受到重视，成为肿瘤基因治疗的主要载体之一。
As the newly emerging vector, recombinant adenovirus is being given more attention and has become the major vector of gene therapy because of its high rate of transfection and expression.
目的：构建趋化因子受体c CR 5,CXCR4双靶区反义rna重组载体并获取重组腺病毒以用于抗hiv1基因治疗的研究。
AIM: to construct the recombinant adenoviral vector carrying antisense RNA to chemokine receptors CCR5 and CXCR4 and to obtain recombinant adenovirus, which will be used to resist HIV 1 infection.
目的：构建趋化因子受体c CR 5,CXCR4双靶区反义rna重组载体并获取重组腺病毒以用于抗hiv1基因治疗的研究。
AIM: to construct the recombinant adenoviral vector carrying antisense RNA to chemokine receptors CCR5 and CXCR4 and to obtain recombinant adenovirus, which will be used to resist HIV 1 infection.
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