利用胰酶-乙二胺四乙酸、核酸酶处理猪心瓣膜制备脱细胞支架。
Decellularized porcine heart valves were prepared by using Trypsin-ethylenediamine tetraacetic acid and DNase.
结果：脱细胞支架细胞成分完全去除，胞外基质保存完好；
RESULTS: The cellular components of heart valves were completely removed and the structure of extracellular matrix was well reserved.
目的为体外构建组织工程瓣提供脱细胞的同种生物瓣支架材料，并建立相应的技术方法。
Objective To provide the scaffold material of decellularized homograft bioprosthetic valve (HBV)for establishing of tissue engineering cardiac valve in vitro, and related technique.
目的：建立成骨细胞-脱钙骨支架复合物，观察其诱导成骨能力，从而探寻组织工程化骨的体外构建方法。
Objiective to probe into the methods of tissue-engineering bone construction in vitro by building osteoblasts-decalcification bone scaffolds complex and observing its ability of inducing osteogenesis.
目的：制备人关节软骨脱细胞基质，为进一步研究关节软骨基质作为细胞外支架材料提供方法学资料。
OBJECTIVE:To prepare human articular cartilage acellular matrix so as to provide a methodological basis for further study of articular cartilage acellular matrix as cell scaffold materials.
本实验进行脱细胞骨基质制备，去除细胞成分，并进行形态学观察，为骨组织工程的新型天然支架材料提供依据。
This experiment prepares ACEM by removing cell components and carries morphologic study to provide foundation for new natural supporting material of bone tissue engineering.
目的：利用软骨微粒脱细胞基质这种新型支架材料及纤维蛋白胶，体外构建可塑形及具有良好生物相容性的组织工程化软骨。
AIM: To establish engineered cartilage with good biocompatibility and plasticity in vitro with fibrin glue and a new material of stent made by cartilage microparticle acellular tissue matrix (CMACTM).
目的探讨脱细胞基质、胶原蛋白基质网架、胶原凝胶的不同支架材料上构建人工真皮的可行性。
Objective To build artificial dermis by using the acellular dermis matrix (ADM), collagen membrane and collagen gel as scaffolds.
评价脱细胞脊髓支架的免疫原性，为其在组织工程中的应用奠定理论基础。
To investigate the immunogenicity of the acellular spinal cord scaffold and to provide theoretical basis for its further application in tissue engineering.
制备领下腺脱细胞基质支架材料的浸提液，通过急性全身毒性试验，分析脱细胞后的生物衍生支架材料对机体的毒性作用。
The extracting liquid of submandibular gland acellular matrix scaffold material was made, and its toxicity effect to the body was analyzed by acute systemic toxicity test.
目的对已成功制备的以脱细胞犬动脉为基质的血管支架进行体外再细胞化研究。
Objective To recellularize a novel tissue engineered blood vessel (TEBV) scaffold based on decellularized canine aorta which we had successfully constructed.
天然胶原类组织，经过脱细胞处理后，降低免疫原性，可作为组织工程的支架材料，本文就脱细胞的方法以及无细胞胶原基质的研究进展作一综述。
As scaffold, nature collagen must be decellularized to decrease its immunity. The methods of decellularization and the progress of study on acellular collagen matrix are reviewed in this article.
目的探索以异体软骨微粒脱细胞基质为支架构建组织工程化软骨。
Objective To construct tissue engineered cartilage using cartilage microparticle acellular tissue matrix(CMACTM)as scaffold.
目的：拟验证脐静脉内皮细胞低密度种植于脱细胞猪动脉支架实现血管内皮化的可行性。
OBJECTIVE: To explore endothelialization of low density human umbilical vein endothelial cell (HUVEC) seeded on decellularized porcine carotid arteries scaffold.
方法：十二烷基硫酸钠和脱氧胆酸钠联合处理获取脱细胞猪动脉支架。
METHODS: Decellularized scaffold was prepared by treating fresh porcine artery and heart valves with sodium deoxycholate and sodium dodecyl sulfate.
目的：探讨脱细胞腮腺支架材料的制备方法，制备涎腺组织工程的理想支架材料。
Objective: To prepare the acellular scaffold of parotid gland stroma with the lower antigenity and native structure for tissue engineering.
结果实际完成观察的关节数为44个，其中空白对照组9个，脱细胞软骨支架组11个，静态培养细胞支架组18个，生物反应器培养细胞支架组6个。
Results Observation was completed only for 44 joints, with 9 ones in the blank group, 11 in the simple scaffold group, 18 ones in the static culture group, and 6 ones in the bioreactor culture group.
结果实际完成观察的关节数为44个，其中空白对照组9个，脱细胞软骨支架组11个，静态培养细胞支架组18个，生物反应器培养细胞支架组6个。
Results Observation was completed only for 44 joints, with 9 ones in the blank group, 11 in the simple scaffold group, 18 ones in the static culture group, and 6 ones in the bioreactor culture group.
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