离心机将脂肪细胞从干细胞和再生细胞中分离出来,将它们浓缩成一个小球,再萃取出来。
A centrifuge separates the fat cells from the stem and regenerative cells, concentrating them into a pellet, which is then extracted.
››然后,通过离心将可再生的干细胞从脂肪细胞中分离出来,将其浓缩成微粒状,再萃取出来。
Next, a centrifuge separates the fat cells from the stem and regenerative cells, concentrating them into a pellet, which is then extracted.
其中一半的脂肪细胞是用来填补缺损造成的空缺,另一半则用于分离可再生的干细胞。
Half the fat is used to create the volume needed to fill the divot and half is processed to isolate stem and regenerative cells.
结论:从未退变的椎间盘分离的纤维环细胞能在体外分化为脂肪细胞、格根包尔氏细胞、软骨细胞、神经元及内皮细胞。
Conclusions Anulus fibrosus cells isolated from nondegenerative intervertebral discs can differentiate into adipocytes, osteoblasts, chondrocytes, neurons, and endothelial cells in vitro.
然后,通过离心将可再生的干细胞从脂肪细胞中分离出来,将其浓缩成微粒状,再萃取出来。
Next, a centrifuge separates the fat cells from the stem and regenerative cells, concentrating them into a pellet, which is then extracted.
近年研究表明,运用相似的分离方法,可从人、小鼠、大鼠、兔和猪等物种脂肪组织中分离获得脂肪间充质干细胞。
Recent researches have demonstrated the potential ability of adipose mesenchymal stem cells (AMSCs) which were harvested from human, mouse, rat, rabbit and pig adipose tissue, respectively.
从乌龙茶中分离促进脂肪分解的活性物质并检查在脂肪细胞中对去甲肾上腺素诱导脂肪分解的作用。
The effects of various active fractions isolated from oolong tea on norepinephrine-induced lipolysis were examined in isolated fat cells.
目的:分离培养人脂肪基质干细胞,鉴定其表型。
AIM: To isolate and culture human adipose tissue derived stem cells, and identify their phenotype.
大量的细胞可以很容易和迅速地分离出脂肪,从而迅速和容易获得大量的细胞。
A large number of cells can be easily and quickly isolated from fat, thus opening the potential for large Numbers of cells to be quickly and easily obtained.
结果:(1)成功分离并培养人前脂肪细胞,观察了它的增殖和分化过程。
Results: (1) We successfully separated and primary cultured the human preadipocyte, and observed the proliferation and differentiation process of preadipocyte.
巴西的研究小组发现,在活体中人类输卵管间质干细胞能轻易的分离和扩展,能分化成肌肉、脂肪、软骨和骨骼细胞株。
The Brazilian team found that human fallopian tube mesenchymal stem cells could be easily isolated and expanded in vitro, and are able to differentiate into muscle, fat, cartilage and bone cell lines.
我们从四个健康的供体分离人脂肪间充质干细胞。
We isolated human adipose tissue-derived MSC from four healthy donors.
本文综述了脂肪来源间充质干细胞的分离培养、生物学特性及其应用等进展,并对其研究前景进行了探讨。
Based on the progress in this field, this review focuses on ADSCs' isolation, culture, biological characteristics and applications, as well as their research prospect.
骨髓基质干细胞是一种由骨髓中分离获得的具有多种分化潜能的间质干细胞。在体外培养条件下,它可以分化为成骨细胞、软骨细胞、脂肪细胞、甚至于成肌细胞。
Bone marrow stromal stem cells isolated from bone marrow are the special mesenchymal stem cells, which can be differentiated in culture into osteoblasts, chondrocytes, adipocytes, and even myoblasts.
目的:探讨人脂肪源干细胞在体外分离培养扩增的方法及向成骨细胞诱导分化的能力。
OBJECTIVE: to establish a method for the isolation and culture of human ADSCs and to explore the ability of osteogenic differentiation.
本文首先用多次联合消化脂肪组织的方法分离培养脂肪干细胞,通过换液的方法去除红细胞,减小了红细胞裂解液对细胞的损伤。
Human ADSCs were isolated firstly in this study with improved method, through the exchange of the cell medium to remove the red blood cells, reducing the damage of cells.
结果与结论:体外分离培养的脂肪源干细胞生长稳定,扩增速度快。流式细胞仪检测结果显示其高表达干细胞相关抗原。
RESULTS and CONCLUSION: ADSCs could be expanded steadily in vitro and flow cytometry revealed high expression of stem cell-associated markers.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养,保留贴壁细胞传代,分析脂肪间充质干细胞的纯度,观察细胞生长情况,检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养,保留贴壁细胞传代,分析脂肪间充质干细胞的纯度,观察细胞生长情况,检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
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