提示可采用生化测定胶原在同种软骨移植后不同时期的含量变化来评价异体软骨移植后的结构和弹性变化。
The study suggests that the changes in cartilage collagen content may help us to assess the cartilage structure and the elasticity after a cartilage allotransplantation.
测定脱细胞血管基质中胶原蛋白含量,检测血管脱细胞血管基质的伸长比、极限应力。
The collagen protein level of acellular vascular matrix was evaluated, the elongation ratio and ultimate stress were assayed as well.
取背部皮肤,用分光光度法测定小鼠皮肤中胶原蛋白的含量。
The contents of collagen in mice back skin were measured by photometry.
方法:采用生化法测定胶原含量、胶原类型及胶原酶的变化以反映固定3月及6月后胶原的变化。
Methods: the collagen content the types of collagen and the activity of collagen enzyme were surveyed by biochemical technique at the 3 rd, 6 th months postoperatively.
病理染色图象分析测定左室腔周长和面积、单位面积内心肌细胞核数、单位面积内胶原的含量;
Pathological staining and image analysis were used to determine the perimeter and the area of left ventricular cavity, and myocardial nuclei number and collagen content per unit area;
方法:羟脯氨酸法、间接酶联免疫吸附法(ELISA)测定博莱霉素A6(BLMA6)诱发的小鼠、大鼠肺纤维化模型肺组织胶原含量及合成,阿利新蓝法测定其糖胺多糖(GAG)含量;
METHODS: Hydroproline determination and ELISA were used for measuring the content of collagen, Alcian blue assay for BLM-A6 induced glycosaminoglycan (GAG) in the lung tissues (rats, mice).
方法:羟脯氨酸法、间接酶联免疫吸附法(ELISA)测定博莱霉素A6(BLMA6)诱发的小鼠、大鼠肺纤维化模型肺组织胶原含量及合成,阿利新蓝法测定其糖胺多糖(GAG)含量;
METHODS: Hydroproline determination and ELISA were used for measuring the content of collagen, Alcian blue assay for BLM-A6 induced glycosaminoglycan (GAG) in the lung tissues (rats, mice).
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