方法：采用全胚胎体外培养技术。
本研究首次将CZB培养液应用于昆明小鼠早期胚胎的培养，建立了一个可行的胚胎体外培养的新方法。
In this study, the CZB medium was used to culture IVF embryos of KM strain mouse, a practical new method of culturing mouse embryos was established.
本文对禽类胚胎生物工程技术，包括禽类胚胎体外培养技术、禽类嵌合体技术和禽类基因转移技术的研究进行了综述。
The studies on biological engineering technology of embryo in birds, including the in vitro culture, chimera and transgenic birds are summarized in the essay.
免疫活性肽(ICP)和表皮生长因子(EGF)是两种生物活性因子，ICP对牛卵母细胞体外成熟及胚胎体外培养的影响还未见报道。
Immunocompentent peptide (ICP) and epidermal growth factor (EGF) are two bio - active factors, the effect of ICP on in vitro maturation of bovine oocytes and development of embryos not being reported.
目的：对不同发育阶段的人胚胎原始生殖细胞（PGC）进行定位并比较其体外培养的差异。
Objective: To study the localization and in vitro culture of human embryonic primordial germ cell(PGC) during different stages of embryogenesis.
目的寻求一种体外稳定培养早期胚胎的方法。
Objective To find out a stable way of in vitro culture of early embryo.
早期胚胎发育过程当中伴随着大量的发育异常的细胞，尤其在体外培养过程中更为普遍。
During the preimplantation embryo development, we can find many abnormal cells especially in the process of in vitro culture.
哺乳动物胚胎在体外培养中普遍存在早期发育阻滞的现象。
The early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro.
试验研究了培养液中血清的添加时间和添加浓度及不同培养体系对黄牛孤雌胚胎体外发育潜力的影响。
The effects of fetal bovine serum (FBS) addition time, concentration and different culture systems on the developmental potentiality of bovine parthenogenetic embryos were observed.
实验以ICR小鼠体外培养的囊胚及孵化胚胎为材料，比较两种方法对小鼠胚胎细胞计数的效果。
The experiment was blastocyst and hatch blastocyst of ICR mouse culture in vitro, and the effect of two methods to the cell counting of mouse embryo was compared.
目的优化猪胚胎体外生产培养系统。
Objective To optimize systems of pig embryo in vitro production.
目的研究低浓度氟环境对体外培养的胚胎鼠颅骨成骨细胞中的各类非胶原蛋白和胶原蛋白的表达。
Objective the expression of various non-collagenous proteins and collagen protein on the rat fetal calvarial osteoblast cultured in low doses fluoride condition in vitro had been studied.
目的研究丙烯腈（ACN）对体外培养的大鼠胚胎脊髓神经细胞增殖分化的影响。
Objective To explore the effects of acrylonitrile (ACN) on proliferation and differentiation of spinal cord nerve cells of rat embryo in vitro.
目的研究人胚胎海马神经干细胞体外长期培养的条件和其在自主分化条件下的分化能力和分化特点。
Objective To study the conditions of in vitro culture and the features of differentiation of neural stem cells from human embryonic hippocampus.
哺乳动物早期胚胎的体外培养是胚胎工程技术中一个重要的环节。
The technique of early embryos culture in vitro is an important tache to the mammal embryos engineering technology.
猪卵母细胞的体外成熟培养、成熟卵母细胞的孤雌激活和胚胎培养是胚胎生物工程技术的主要环节。
The culture of porcine oocytes maturation in vitro, parthenogenetic activation of MII porcine oocytes and embryo culture are main technology of embryo biology engineering.
目的探讨胚胎大鼠神经干细胞的体外培养条件及外源基因的表达效率。
Objective to explore the culture conditions for the neural stem cells from embryonic rat cortices and to test their efficacy of expressing exogenous gene.
方法采用大鼠胚胎中脑神经元细胞进行体外培养。
Methods Using dissociated cell culture of rat embryo in vitro.
目的：观察脑源性神经生长因子（BDNF）和神经营养因子-3（NT-3）单独及联合应用对体外培养胚胎大鼠脊髓神经元存活和生长发育的影响。
Objective:To study the individual and joint effects of BDNF and NT-3on the sur-vival and growth of neurons from the spinal cord of embryonic rat.
目的采用体外培养方式，探讨日本血吸虫未成熟卵可溶性抗原（SIEA ）免疫血清的抗卵胚胎发育作用。
Objective Study the anti embryonation of Schistosoma japonicum eggs by soluble immature egg antigen (SIEA) immunized serum in vitro.
方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。
The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.
目的：为保持胚胎干细胞的高度未分化状态，饲养层细胞成为胚胎干细胞体外培养中不可缺少的条件。
AIM: Feeder layer cells are the necessary condition to prevent the differentiation of embryonic stem cells cultured in vitro.
目的：建立大鼠胚胎视网膜祖细胞体外培养方法并观察其分化特性。
Objective: to isolate and incubate neural progenitor cells from rat embryonic retina and to explore their characteristics of differentiation.
据报道，ES细胞目前已有在体外培养两年，连续传代达450次以上，而胚胎生殖干细胞传代次数最多可达70~80次。
According to reports, ESC culture in vitro was more than two years and attained 450 serial passages. Embryonic germline ESC attained 70~80 passages at most.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
以CZB为基础培养液，培养小鼠4、8 -细胞胚胎单卵裂球，研究葡萄糖、牛磺酸和猪输卵管上皮细胞共培养在其体外发育中的作用。
CZB medium was used as a base medium to study the function of taurine, glucose and porcine oviductal epithelial cells system in the culture of blastomeres isolated from mouse 4? 8-cell embryos.
通过培养条件的优化，使烟草合子在体外通过器官发生途径与胚胎发生途径两种方式再生可育植株，从而创建了烟草合子的体外胚胎发生系统。
As a result, an in vitro zygotic embryogenesis system was build up, in which single tobacco zygotes could develop into fertile plants via embryogenesis or organogenesis under optimal condition.
通过培养条件的优化，使烟草合子在体外通过器官发生途径与胚胎发生途径两种方式再生可育植株，从而创建了烟草合子的体外胚胎发生系统。
As a result, an in vitro zygotic embryogenesis system was build up, in which single tobacco zygotes could develop into fertile plants via embryogenesis or organogenesis under optimal condition.
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