目的探讨青石棉致人胚肺成纤维细胞(HEPF)增殖的细胞周期变化的机制。
Objective To explore the mechanism of cell proliferation cycle change of human embryonic pulmonary fibroblasts (HEPF) induced by crocidolite.
应用氯化镉致人胚肺成纤维细胞恶性转化实验、染色体分析、流式细胞术对氯化镉的致癌机理进行研究。
The mechanism of carcinogenesis induced by CdCl 2 was studied through experiments of the malignant transformation of human embryo lung fibroblast in vitro, chromosome analysis and flow cytometry.
目的：研究依他酸（EA）和卡西霉素（Cal）对人胚肺成纤维细胞脱氧核糖核酸和胶原合成的影响。
AIM: To study the effects of egtazic acid (EA) and calcimycin (Cal) on the synthesis of DNA and collagen in cultured human lung fibroblasts (HLF).
方法从培养的人胚肺成纤维细胞(WI- 38)中提取总rna，经rt -PCR获得人破骨细胞抑制因子基因。
METHODS Total RNA was extracted from human embryonic lung fibroblast cells (WI 38) and the whole length gene of human OPG was obtained by RT PCR.
同时以端粒酶阳性的永生化人胚肾成纤维细胞HEK293作为阳性对照，以端粒酶阴性的人胚肺成纤维细胞HELF作为阴性对照。
Immortalized human embryonic kidney cell line HEK293 and the human embryonic lung fibroblasts HELF were used as positive control and negative control, respectively.
结果表明，体外培养人胚肺二倍体成纤维细胞成活至52代，而含0。2%黄芪制取液的培养基使细胞成活至77代。
The results showed that heldf cultured in vitro lived up to52generations while the culture medium containing0.2% amb extract made the cells live up to77genera-tions.
目的观察衰老的人胚肺二倍体成纤维细胞(2bs)对烷化剂甲磺酸甲酯(MMS)诱导的DNA损伤的应答。
Objective to explore the cellular response of human senescent diploid fibroblasts (2bs) to the DNA damage caused by methyl methanesulfonate (MMS).
结果表明，体外培养人胚肺二倍体成纤维细胞成活至52代，而含0.2%黄芪制取液的培养基使细胞成活至77代。
The results showed that HELDF cultured in vitro lived up to 52 generations while the culture medium containing 0.2% AMB extract made the cells live up to 77 generations.
而消化的人胚肺组织中细胞形态不一，经继续培养4～5代后，培养物完全由成纤维细胞构成。
The shapes of human embryonic lung fibroblasts which were isolated by enzyme digestion were differ, and the culture was all composed of fibroblasts after subculturing for 4-5 generations.
而消化的人胚肺组织中细胞形态不一，经继续培养4～5代后，培养物完全由成纤维细胞构成。
The shapes of human embryonic lung fibroblasts which were isolated by enzyme digestion were differ, and the culture was all composed of fibroblasts after subculturing for 4-5 generations.
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