为了探讨人胚神经干细胞体外培养条件和分化情况，摸索出一种切实可行的获得较纯、多潜能人胚神经干细胞的方法。
This is a study on the cultivation condition in vitro and differentiation of neural stem cells from human embryonic brain in order to find a way to get purified multipotential neural stem cells.
目的探讨人胚神经干细胞体外培养的条件和分化情况，以摸索出一种切实可行的能获得较纯且多潜能人胚神经干细胞的方法。
Objective to study the cultivation condition in vitro and differentiation of neural stem cells from the embryonic human in order to find a way to get purified multipotential neural stem cells.
方法从自然流产的孕10 ~13周的人胚脑组织中分离、培养神经干细胞。
Methods human neural stem cells were separated from 10 ~ 13 weeks brains of human embryo and were cultured and induced to differentiate.
目的从大胚龄人胚脑中分离培养并鉴定神经干细胞。
Objective To isolate and culture human neural stem cells from elder embryonic brain.
用鸡胚骨骼肌提取液，诱导神经干细胞向胆碱能神经元方向分化。
Embryonic chick skeletal muscle extract was used to induce the differentiation of the neural stem cells into cholinergic neurons.
结果胚鼠室管膜源性神经干细胞在相应培养条件下呈现出神经干细胞快速增殖，形成由多细胞组成的细胞球（神经球）；
Results The NSCs derived from the fetal rat ependyma were observed to rapidly proliferate to form some cellular spheres or neural spheres consisting of NSCs.
方法：从自然流产的孕9～15周的人胚脑组织中分离、培养神经干细胞。
Methods: HNSCs were separated from brains of 9-15-week human embryo, cultured and induced to differentiate.
方法：从自然流产的孕9～15周的人胚脑组织中分离、培养神经干细胞。
Methods: HNSCs were separated from brains of 9-15-week human embryo, cultured and induced to differentiate.
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