其内容包括：（1）胚性愈伤组织的诱导与保存；
The substance are consists of: 1. initiation and maintenance of embryogenic calli;
结果表明，胚性愈伤组织是棉花染色体观察的最佳材料。
The results indicated that embryogenic callus was the best material for observed chromosome.
而非胚性愈伤组织质地松散有水渍状、表面粗糙、多呈白色。
On the contrary, the non embryogenic callus was loose in texture, rough in surface, watery and white in color.
胚性愈伤组织转移到体细胞胚诱导培养基中可诱导出体细胞胚。
Somatic embryos could be induced after the embryogenic calli were transplanted on somatic embryo induction medium.
适宜诱导大蒜胚性愈伤组织且生长正常的外植体为茎尖和根尖。
Results indicated:1) Induction of somatically embryogenic callus was best when shoot and root tips were used as explants.
长期继代培养的柑橘胚性愈伤组织由胚性细胞和非胚性细胞组成。
In Citrus, the embryogenic calli consisted of embryogenic and non-embryogenic cells.
结果表明，龙眼胚性愈伤组织中内源激素含量比非胚性愈伤组织高；
The results showed that the levels of endogenous phytohormones in embryogenic calli were higher than those in non-embryogenic calli.
测定了龙眼胚性愈伤组织体胚发生过程中若干生物大分子的动态变化。
The dynamic changes of some biomacromolecules were measured in the process of somatic embryogenesis from embryogenic callus in longan(Dimocarpus longan Lour. ).
非胚性愈伤组织细胞的ATP酶定位于液泡，与液泡的水解功能有关。
In the cells of non-embryogenic calli, ATPase located in vacuoles and involved in the function of the hydrolysis of vacuole.
从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。
Many embryogenic calli were produced by subculture of calli induced from mature embryo of rice.
结果表明，非胚性愈伤组织和胚性愈伤组织在表面结构上存在着明显的差异。
The results showed that the structure differences between embryogenic callus and non-embryogenic callus were distinct.
由此看来，胚性愈伤组织的诱导和保持，可能还与各种激素的协同作用有关。
By this token, induction and subculture of embryonic calli should be related with cooperative effects among different hormones.
以红姜茎尖薄片胚性愈伤组织为材料建立了胚性细胞悬浮系，并获得了再生植株。
Regenerated plantlets were obtained in embryogenic cell suspension culture from embryogenic callus of stem slice in Hedychium coccineum Buch-Ham.
体细胞胚起源于胚性愈伤组织中含单宁的细胞包围的单个胚性母细胞，属于单细胞起源。
The somatic embryo originated from single embryonic original cell of embryonic callus tissues, which was surrounded by tannin cells, that belonged to single cell origination.
小麦成熟胚愈伤组织的诱导率、生长率及胚性愈伤组织发生率的基因型效应有显著差别。
The effect of genotype on the rate of induction, growth and embryogenic callus initiating were different significantly.
该培养基适于培养大豆等豆科植物未成熟子叶原生质体，通过胚性愈伤组织，经胚胎发生途径。
This callus can differentiate somatic embryo and regenerate plant on the culture medium somatic embryo differentiation.
试验还发现2,4 - D起主要作用，但是BA、KT的加入可显著提高胚性愈伤组织的诱导率。
Furthermore 2, 4-d play an important role in induction of EC, but adding BA and KT can improve IR.
主要结果如下：1、起初诱导的胚性愈伤组织及处于球形期的体胚，再生能力强于其它发育时期的体胚。
The main results were described as follows: 1 Initial inducing embryogenic callus and globular embryos had super regeneration capacity than other stage embryos.
结果表明，不同基因型荔枝花药胚性愈伤组织诱导率存在显著差异，胚性愈伤组织倍性检测结果也不同。
The ploidy detection indicated that the genotypes performed marked difference in the inducing rates of anther embryogenic calli and produced different results in ploidy detection of embryogenic calli.
大麦成熟胚的初代愈伤组织是水质、柔软的非胚性愈伤组织，在继代培养中能以一定频率出现胚性愈伤组织。
The primary callus induced from barley mature embryos is soft, watery and nonembryogenic, but after subculture it can produce compact embryogenic callus with a certain frequency.
外源生长素和细胞分裂素是诱导离体培养细胞分化与增殖所必需的，2，4-D是诱导胚性愈伤组织的重要激素。
The exogenous auxin and cytokinin are necessary to induced differentiation and proliferation of cells of culture in vitro. 2,4-D is an important hormone of induced embryogenic calluses.
但是胚性愈伤组织的ABA含量却低于非胚性愈伤组织，且胚性愈伤组织诱导率与胚性愈伤组织IAA含量负相关。
It was found, however, that ABA content of embryonic calli was lower than non-embryonic calli, and induction rate of embryonic calli was correlated negatively with IAA content of embryonic calli.
对棉花体细胞胚胎发生和植株再生基因型范围的拓宽进行了研究，有望得到丰产抗病棉花品种农大94 - 7的胚性愈伤组织。
To broad the number of regenerable cotton species, a new high yield and disease resistant variety, Nongda94-7 was studied, and Embryogenic callus of it could be induced for the future.
以红豆草无菌苗胚轴为外植体诱导愈伤组织，在此基础上建立了“胚性细胞系”和“非胚性细胞系”。
The hypocotyls of aseptic seedlings of Onobrychis viciaefolia Scop. were used as explants for inducing callus. The embryonic cell line ( E-line) and non-embryonic cell line (NE line) were established.
组织学切片证明所诱导的愈伤组织是胚性组织，其所产生的体胚具有典型的单子叶植物体细胞胚的组织结构。
A plantlet had been obtained. Histological sections proved that the embryos had a typical histological structure of monocot somatic embryos.
组织学切片证明所诱导的愈伤组织是胚性组织，其所产生的体胚具有典型的单子叶植物体细胞胚的组织结构。
A plantlet had been obtained. Histological sections proved that the embryos had a typical histological structure of monocot somatic embryos.
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