目的对钒酸氧化法、改良j - G法、胆红素氧化酶法进行方法学比较，选择胆红素测定的最好方法。
Objective It is to select the best method to determine bilirubin through technology comparison between vanadic acid oxidation method, improved J-G method and bilirubin Oxidase method.
方法胆红素氧化酶突变体i 402g和C457S通过聚合酶链反应获得，并经氨基酸序列测定加以证实。
Methods The BO mutants I402G and C457S were obtained by site-directed mutagenesis and confirmed by amino acid sequence analysis.
方法胆红素氧化酶突变体i 402g和C457S通过聚合酶链反应获得，并经氨基酸序列测定加以证实。
Methods The BO mutants I402G and C457S were obtained by site-directed mutagenesis and confirmed by amino acid sequence analysis.
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