结果：采集样品10件中4件样品检出金黄色葡萄球菌，且肠毒素检测均为阳性。
Results: 4 samples were detected staphylococcus aureus in 10 samples collected and the enterotoxin are all positive.
目的：建立金黄色葡萄球菌肠毒素基因的检测方法。
Methods: Toxin genes were amplified by PCR, and detected by electrophoresis.
目的利用PCR技术，尝试建立特异性引物PCR快速检测肠毒素大肠杆菌的方法。
ObjectiveTo establish the rapid detection method of Enterotoxigenic E. coli(ETEC)by polymerase chain reaction(PCR)technology for characteristic primers.
另外，从分子结构与功能、作用机理、基因检测三方面叙述了新生仔猪etec不耐热肠毒素和耐热肠毒素的研究进展。
Besides, molecular structures, functions, mechanisms and gene detection of both heat-labile and heat-stable enterotoxins of etec in newborn piglets were discussed briefly too.
结果：建立了金黄色葡萄球菌肠毒素基因PCR检测方法。
Results:The typing method for Staphylococccal toxin genes with PCR was developed.
以纯化的重组F41菌毛蛋白作为检测抗原，建立了检测产肠毒素大肠杆菌F41菌毛抗体的间接ELISA方法。
An indirect enzyme-linked immunosorbent assay (ELISA) was developed based on a purified recombinant F41 pili protein of enterotoxigenic Escherichia coli (ETEC).
（in Chinese）汪永禄，王多春，张萍，等。临床患者标本金黄色葡萄球菌肠毒素基因及耐药性的检测分析 [J]。公共卫生与预防医学，2013，24（2）：18-22。
Wang YL, Wang DC, Zhang P, et al. Enterotoxin genes of Staphylococcus aureus of clinical specimens and drug resistance [J]. Journal of Public Health and Preventive Medicine, 2013,24(2):18-22.
（in Chinese）汪永禄，王多春，张萍，等。临床患者标本金黄色葡萄球菌肠毒素基因及耐药性的检测分析 [J]。公共卫生与预防医学，2013，24（2）：18-22。
Wang YL, Wang DC, Zhang P, et al. Enterotoxin genes of Staphylococcus aureus of clinical specimens and drug resistance [J]. Journal of Public Health and Preventive Medicine, 2013,24(2):18-22.
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