目的:建立金黄色葡萄球菌肠毒素基因的检测方法。
Methods: Toxin genes were amplified by PCR, and detected by electrophoresis.
结果:建立了金黄色葡萄球菌肠毒素基因PCR检测方法。
Results:The typing method for Staphylococccal toxin genes with PCR was developed.
结果:散发性霍乱以无毒株为主,质粒及肠毒素基因的携带率极低,但耐药性有所增加。
Results: The sporadic outbreak of vibrio cholera has some characters: including non toxic strain infection, low detection rate of plasmid and ct gene and increase of drug resistance.
方法:采用血清学和分子生物学相结合的方法,研究深圳地区散发不同血清型霍乱弧菌的生化特性、质粒、肠毒素基因携带和耐药状况。
Methods: With serological and molecular techniques, we study chemical property, plasmid, CT gene and drug resistance of sporadic outbreak of vibro cholera in Shenzhen city.
ETEC的肠毒素基因和菌毛蛋白基因对于ETEC的致病性起主要作用,肠毒素基因工程疫苗为预防ETEC引起的腹泻开辟了新的途径。
The enterotoxin gene and fimbrial gene are major reason for the pathogenic character of ETEC. The new method is found in preventing from diarrhea, which is enterotoxin genetic engineering vaccine.
(in Chinese)汪永禄,王多春,张萍,等。临床患者标本金黄色葡萄球菌肠毒素基因及耐药性的检测分析 [J]。公共卫生与预防医学,2013,24(2):18-22。
Wang YL, Wang DC, Zhang P, et al. Enterotoxin genes of Staphylococcus aureus of clinical specimens and drug resistance [J]. Journal of Public Health and Preventive Medicine, 2013,24(2):18-22.
目的对金黄色葡萄球菌肠毒素A基因(SEA)进行克隆、鉴定与表达,为制备单抗、诊断试剂及进一步深入研究SEA的功能奠定基础。
Objective To clone, identify, and express the staphylococcal enterotoxins A(SEA) gene from Staphylococcus aureus in order to prepare monoclonal antibody and diagnostic reagent.
另外,从分子结构与功能、作用机理、基因检测三方面叙述了新生仔猪etec不耐热肠毒素和耐热肠毒素的研究进展。
Besides, molecular structures, functions, mechanisms and gene detection of both heat-labile and heat-stable enterotoxins of etec in newborn piglets were discussed briefly too.
作者综述了干扰素基因、NRAMP1基因、肠毒素大肠杆菌、MHC基因等候选基因的研究进展,以期为抗病育种提供借鉴。
This article reviewed research progress of IFN gene NRAMP1 gene ETEC MHC gene which are candidate gene of disease resistance pig.
本实验室使霍乱肠毒素(CT)B亚单位基因在大肠杆菌中获得表达,并能分泌到胞外。
The recombinant plasmid pMM2 contained cholera enterotoxin B sub-unit gene CT-B were expressed in E. coli strain.
本实验室使霍乱肠毒素(CT)B亚单位基因在大肠杆菌中获得表达,并能分泌到胞外。
The recombinant plasmid pMM2 contained cholera enterotoxin B sub-unit gene CT-B were expressed in E. coli strain.
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