利用胶原酶灌注梯密度离心分离肝星状细胞，用有限稀释法建立大鼠肝星状细胞株ig12。
Hepatic stellate cell was isolated with collagenase perfusion and ladder density centrifuge. Hepatic stellate cell line IG12 was cloned with limited diluted method.
方法采用HSC - T6肝星状细胞系作为活化的肝星状细胞的研究模型。
Methods HSC-T6 hepatic stellate cell line was chosen as the study model of the activated hepatic stellate cells.
方法采用HSC-T6肝星状细胞系作为活化的肝星状细胞的研究模型。
Methods HSC-T6 rat hepatic stellate cell line was chosen as the study model of the activated hepatic stellate cells.
目的：在精密肝切片中利用乙醛激活肝星状细胞，建立一种星状细胞激活的体外模型，为研究和筛选阻抑肝纤维化发生的药物奠定基础。
AIM: To activate hepatic stellate cells (HSC) in vitro in precision-cut liver slices (PCLS) stimulated by acetaldehyde for studying and screening anti-fibrotic drugs.
目的：在精密肝切片中利用乙醛激活肝星状细胞，建立一种星状细胞激活的体外模型，为研究和筛选阻抑肝纤维化发生的药物奠定基础。
AIM: To activate hepatic stellate cells (HSC) in vitro in precision-cut liver slices (PCLS) stimulated by acetaldehyde for studying and screening anti-fibrotic drugs.
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