本论文应用基因工程的手段克隆、表达并纯化了人的肝再生增强因子基因。
This dissertation USES the gene engineering method to clone, express and purifies the human ALR gene.
结论从2周龄大鼠肝组织中成功克隆肝再生增强因子基因,并获得了原核细胞高效表达。
ConclusionThe augementer of liver regeneration gene was cloned from the liver tissue of 2 week old SD rat and the protein encoded by the gene was expressed in the prokaryotic cells.
目的筛选肝再生增强因子重组质粒基因治疗时较优的载体、途径和剂量及三者的最佳组合。
Objective To select an optimal combined set of carrier, introducing route and dose of recombinant plasmid of augmenter of liver regeneration (ALR) for ALR gene therapy.
目的观察重组大鼠肝再生增强因子(alr)对肝星状细胞株ig12增殖及细胞外基质合成的影响,探讨其抗肝纤维化作用的可能机制。
Objective To observe the effects of recombinant augmenter of liver regeneration (ALR) on the proliferation of hepatic stellate cell line IG12 and ECM synthesis.
目的观察重组大鼠肝再生增强因子(alr)对肝星状细胞株ig12增殖及细胞外基质合成的影响,探讨其抗肝纤维化作用的可能机制。
Objective To observe the effects of recombinant augmenter of liver regeneration (ALR) on the proliferation of hepatic stellate cell line IG12 and ECM synthesis.
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