RNA转录后加工的主要步骤有5端加帽，剪接和3端多聚腺苷酸化。
Posttranscriptional mRNA processing includes 5 '-capping, splicing and poly-adenylation at the 3' end.
结果多聚腺苷酸化信号缺陷病毒可以在PA317病毒包装细胞形成病毒颗粒；
Results polyadenylation signal-deficient retroviruses could be packaged by PA317 packaging cells.
通过改变前体mrna的切割位点和聚腺苷酸化位点而从一个编码区产生不同大小mrna的能力。
The ability to make mRNAs of varying sizes from one coding region, by altering the site of pre-mRNA cleavage and polyadenylation.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
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