单细胞反转录酶聚合链式反应检测胰岛素促进因子基因实验中，选择12个单个胰腺干细胞进行检测，另取12个单个未分化的干细胞作为对照。
In the detection of IPF1 gene with single cell RT-PCR, 12 single pancreatic stem cells were detected, and another 12 undifferentiated single pancreatic stem cells were taken as controls.
聚合酶链式反应可使DNA自我复制，产生大量的DNA样品可供科学家进行研究。
Polymerase helps coax DNA to replicate itself, leaving researchers with large numbers of samples to work with.
首先看看他的成就：他发明了一项叫做聚合酶链式反应的技术，这项技术对于生物学的意义就如同晶体管对于计算机的意义。
First his accomplishments: he invented a process called PCR, which is every bit as important to biology as the transistor is to computers.
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
血清学检测是针对麻风杆菌特异性式碳酸糖酯抗体进行，此外聚合酶链式反应（PCR）也可用于检测，但对于早期诊断并不可靠。
Serology aimed at M leprae-specific phenolic glycolipidantibodies and polymerase chain reaction may also be used but fail to reliablydetect early disease.
TED大会提到，Muliis发明了聚合酶链式反应技术的这一成就看起来可以盖过其他一些事。
Ted mentions that Mullis invented PCR and surfs and seems to skim over a few other things.
聚合酶链式反应仪(PcR仪)是分子生物学的基本仪器。
The polymerase chain reaction (PCR) machine is a basic instrument in molecule biology.
形成聚合物的最普通方式是靠两种主要的增长反应，习惯上分别称做缩合（逐步反应）过程和加成（链式反应）过程。
Polymers are most commonly formed by two main types of growth reaction traditionally referred to us condensation (step reaction) and addition (chain reaction) processes.
目的评估用逆转录聚合酶链式反应(RT PCR)方法检测胃癌腹腔微转移的临床病理意义。
Objective to evaluate the clinicopathologic significance of the detection of peritoneal micrometastases in gastric cancer by reverse transcriptase-polymerase chain reaction (RT-PCR).
P CR芯片是进行DNA聚合酶链式反应的芯片，温控部分的搭构是决定整个PCR芯片性能、尺寸、集成化的重要组成部分。
PCR-chip is a function biochip for DNA polymerase chain reaction. The temperature control section is so important that it decides the performance, dimension, integration of the whole chip.
“聚合酶链式反应”的简称，一种可在血液或骨髓中检测到癌细胞标志物存在的灵敏的实验室试验。
PCR the short name for "polymerase chain reaction," a sensitive lab test that can measure the presence of cancer cell-markers in the blood or marrow.
实验室鉴定方法包括聚合酶链式反应、限制酶片段长度多态性分析以及生物芯片技术等。
In laboratories, the methods mainly include polymerase chain reaction, restriction fragment length polymorphism, and biochip technology. The methods mentioned above all have their merits and defects.
本研究利用聚合酶链式反应技术，成功地克隆了枯草芽孢杆菌缺陷型原噬菌体PBSX阻遏基因及其温度敏感型等位基因。
The repressor gene and its allele-a temperature sensitive mutant have been cloned from the defective prophage PBSX of Bacillus subtilis by means of PCR technique.
目的探讨聚合酶链式反应变性及退火温度与产物假阴性之间的关系。
Objective to explore the relationship of temperatures for denaturalization and annealing to the false negative PCR result.
介绍了分子生物学前沿技术聚合酶链式反应(PCR)的原理、方法及应用。
The principle, method and application of Polymerase Chain Reaction (PCR) was introduced in this paper.
检测橘蚜携带CTV的分子生物学反转录-聚合酶链式反应技术已建立，并已应用于检测橘蚜等蚜虫的单虫带毒情况。
Reverse transcription polymerase chain reaction technique for the detection of CTV in a single aphid or aphids has been developed.
双轮回‘聚合酶链式反应’(PCR)和‘反向转录酶-聚合酶链式反应’(RT - PCR)被用来检测基因CYP1A1的转录水平。
A double - round reverse transcriptase - coupled polymerase chain reaction (RT - PCR) was applied to detect the CYP1A1 transcript.
目的运用依赖随机化末端连接物聚合酶链式反应(RDPCR)技术检测环境中混合污染物的DNA损伤作用。
Objective To detect DNA lesions induced by complex pollutants existing in environment using the technique of randomized terminal linker, dependent PCR (RDPCR).
实验室鉴定方法包括聚合酶链式反应、限制酶片段长度多态性分析以及生物芯片技术等。
In laboratories, the methods mainly include polymerase chain reaction, restriction fragment length polymorphism, and biochip technology.
本发明进一步提供用作分子探针或聚合酶链式反应(PCR)扩增引物的核酸分子，即合成的或天然的寡聚核苷酸。
The present invention further provides nucleic acid molecules for use as molecular probes, or as primers for polymerase chain reaction (PCR) amplification, i. e. synthetic or natural oligonucleotides.
方法提取肿瘤组织总rna，用反转录聚合酶链式反应(RT - pcr)法进行检测。
Methods Total RNAs of tumor tissues were extracted and were detected by RT-PCR.
应用聚合酶链式反应（PCR）、多重PCR方法检测GSTM1和GSTT1在正常人群和结直肠肿瘤患者群体中的基因多态性分布。
The frequencies of GSTM1-null and GSTT1-null genotypes in colorectal tumor and control groups were ascertained by using polymerase chain reaction (PCR) and multiple-PCR techniques.
采用顺序特异性引物聚合酶链式反应（PCR-SSP）DNA分型技术，首次对35例肾移植供受者和4份标准DNA进行HLA-DR2、DR7、DR9基因配型。
HLA-DR2, DR7, DR9 genotyping by polymerase chain reaction with sequence-specific primers (PCR-SSP)was carried out for 35 individuals and 4 cell lines DNA.
方法收集160例临床已确诊的肠癌病人及120例健康体检者血液标本，采用聚合酶链式反应(PCR)技术进行B19DNA检测。
Methods B19DNA in blood samples of 160 clinically confirmed large intestine cancer cases and 120 healthy persons were determined by PCR.
新的快速检测技术有聚合酶链式反应(PCR)技术、伏安型生物传感器、自动微生物检测系统(ams)、改良MRS培养基和三磷酸腺苷(atp)法等，较传统方法迅速、准确。
The new rapid determination techniques were polyase chain reaction method (PCR), volt-ampere biosensor method, automatic microbe system method (AMS), improvement of MRS culture medium and ATP etc.
采用聚合酶链式反应（PCR）技术对来自北京等地区的173份PCV2阳性样本的全长ORF2基因进行了扩增，并对扩增产物进行了限制性片段长度多态性（RFLP）分析。
Whole ORF2 genes of 173 positive clinical PCV2 samples from Beijing and other areas were amplified by PCR and followed by restriction fragment length polymorphism (RFLP) analysis.
基因表达采用逆转录-聚合酶链式反应（RT-PCR）方法。
The mRNA was detected by using reverse transcription-polymerase chain reaction analyses(RT-PCR);
聚合酶链式反应(PCR)技术已在分子生物学、基因测序、医学诊断等方面得到广泛的应用。
Polymerase chain reaction (PCR) technology has been successfully used in clinic diagnostic and modern molecular biology for gene analyzing.
聚合酶链式反应(PCR)技术已在分子生物学、基因测序、医学诊断等方面得到广泛的应用。
Polymerase chain reaction (PCR) technology has been successfully used in clinic diagnostic and modern molecular biology for gene analyzing.
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